Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   H1C74_RS09035 Genome accession   NZ_LR778148
Coordinates   1894675..1896057 (+) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli isolate SC475     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1889675..1901057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1C74_RS09010 deoD 1890689..1891408 (+) 720 WP_000224877.1 purine-nucleoside phosphorylase -
  H1C74_RS22740 - 1891569..1891832 (-) 264 Protein_1762 helix-turn-helix transcriptional regulator -
  H1C74_RS09020 lplA 1891864..1892880 (-) 1017 WP_000105889.1 lipoate--protein ligase LplA -
  H1C74_RS09025 ytjB 1892908..1893552 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  H1C74_RS09030 serB 1893658..1894626 (+) 969 WP_001132955.1 phosphoserine phosphatase -
  H1C74_RS09035 radA/sms 1894675..1896057 (+) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  H1C74_RS09040 nadR 1896078..1897310 (+) 1233 WP_000093810.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  H1C74_RS09045 ettA 1897617..1899284 (-) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=1008284 H1C74_RS09035 WP_001029698.1 1894675..1896057(+) (radA/sms) [Escherichia coli isolate SC475]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1008284 H1C74_RS09035 WP_001029698.1 1894675..1896057(+) (radA/sms) [Escherichia coli isolate SC475]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAACTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGTGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43

Multiple sequence alignment