Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABLV49_RS02245 Genome accession   NZ_CP157675
Coordinates   449334..450899 (-) Length   521 a.a.
NCBI ID   WP_349279995.1    Uniprot ID   -
Organism   Polaromonas hydrogenivorans strain DSM 17735     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 437462..451707 449334..450899 within 0


Gene organization within MGE regions


Location: 437462..451707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLV49_RS02175 (ABLV49_02175) istB 437462..438217 (-) 756 WP_349279989.1 IS21-like element helper ATPase IstB -
  ABLV49_RS02180 (ABLV49_02180) - 438241..438744 (-) 504 Protein_418 IS21 family transposase -
  ABLV49_RS02185 (ABLV49_02185) - 438886..439386 (-) 501 Protein_419 integrase core domain-containing protein -
  ABLV49_RS02190 (ABLV49_02190) - 439428..440504 (-) 1077 WP_349276567.1 IS3 family transposase -
  ABLV49_RS02195 (ABLV49_02195) - 440501..441016 (-) 516 WP_349279284.1 transposase -
  ABLV49_RS02200 (ABLV49_02200) istA 441086..442114 (-) 1029 Protein_422 IS21 family transposase -
  ABLV49_RS02205 (ABLV49_02205) - 442357..443193 (-) 837 Protein_423 IS110 family transposase -
  ABLV49_RS02210 (ABLV49_02210) - 443310..444366 (-) 1057 Protein_424 IS3 family transposase -
  ABLV49_RS02215 (ABLV49_02215) istA 444368..445433 (-) 1066 Protein_425 IS21 family transposase -
  ABLV49_RS02220 (ABLV49_02220) - 446021..446143 (+) 123 WP_349279990.1 hypothetical protein -
  ABLV49_RS02225 (ABLV49_02225) - 446258..446746 (+) 489 WP_349279991.1 hypothetical protein -
  ABLV49_RS02230 (ABLV49_02230) - 446793..447236 (+) 444 WP_349279992.1 hypothetical protein -
  ABLV49_RS02235 (ABLV49_02235) - 447276..447713 (-) 438 WP_349279993.1 putative toxin-antitoxin system toxin component, PIN family -
  ABLV49_RS02240 (ABLV49_02240) - 447716..449140 (-) 1425 WP_349279994.1 THUMP domain-containing protein -
  ABLV49_RS02245 (ABLV49_02245) rpoN/rpoN1 449334..450899 (-) 1566 WP_349279995.1 RNA polymerase factor sigma-54 Machinery gene
  ABLV49_RS02250 (ABLV49_02250) lptB 450910..451707 (-) 798 WP_349279996.1 LPS export ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 521 a.a.        Molecular weight: 57352.42 Da        Isoelectric Point: 4.7305

>NTDB_id=1008046 ABLV49_RS02245 WP_349279995.1 449334..450899(-) (rpoN/rpoN1) [Polaromonas hydrogenivorans strain DSM 17735]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVADDSAPREEFGLDQTDTPVSQDSREFDSATD
SIAINTDTSSVSSQKESETTAEASTEATLEESWEGDGSVESSPDDSEWGGDAPARKNNLDDSDAEASDLTGAHESLQDHL
HRQALALRLSEVDRAALYFLIESLNDDGYLEDSLASLAAGLAHGDLEQTEELEQCFAMALQLLQHMEPAGVGARTLAECL
NLQLLDCKDCEETRAAIAICKQPMELLAKRDFKRLASLCRFSEDAIKGAMAVIARLDPKPGRRFANVERNLIVPDVIVVK
SGRGFKVTLNSDVMPRLRVHDIYANALKQHSGRGSSGPGGGQALQQRLQEARWFIKNIQQRFDTILRVSNAIVERQKNFF
THGELAMRPLVLREIADELGLHESTISRVTTAKYMGTPFGTFELKYFFGSGLGTESGGNASSTAVRALIKQFVASESPKK
PLSDSQISEMLKEQGIECARRTVAKYREALRIAPTNLRKAL

Nucleotide


Download         Length: 1566 bp        

>NTDB_id=1008046 ABLV49_RS02245 WP_349279995.1 449334..450899(-) (rpoN/rpoN1) [Polaromonas hydrogenivorans strain DSM 17735]
ATGAAGCAAGGCCTTTCCCTCCGCGTCTCGCAGCACCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTTTCGACGCTGGAGCTGAGCCAGGAAGTCGAGCAGATGCTTGATGAAAATCCTTTTCTTGAAGTGGCTGACGACTCGG
CGCCGCGCGAAGAGTTTGGACTGGACCAGACCGACACGCCTGTCAGCCAGGACAGCCGCGAATTCGATTCCGCTACGGAT
TCGATAGCTATAAACACAGATACATCAAGCGTAAGCAGCCAAAAAGAGTCTGAAACCACGGCAGAAGCAAGCACCGAAGC
CACACTGGAAGAAAGCTGGGAAGGCGACGGGTCGGTCGAGTCCTCGCCCGATGACAGCGAATGGGGCGGCGATGCGCCGG
CGCGCAAGAACAACCTGGACGACAGCGATGCCGAAGCCTCGGACCTGACCGGCGCCCACGAATCGCTGCAGGACCACCTG
CACCGGCAGGCGCTGGCCCTGCGCCTGTCCGAAGTGGACCGCGCCGCGCTTTACTTCCTGATCGAGTCGCTCAATGACGA
TGGCTACCTCGAAGACAGCCTGGCCTCGCTCGCGGCCGGCCTGGCGCATGGCGACCTGGAGCAGACCGAGGAGCTGGAGC
AGTGCTTCGCCATGGCGCTGCAGTTGCTGCAGCACATGGAGCCGGCCGGCGTCGGCGCGCGCACCCTGGCCGAATGCCTG
AACCTGCAACTGCTCGATTGCAAGGATTGCGAGGAAACCCGCGCCGCCATCGCCATCTGCAAGCAGCCGATGGAACTGCT
GGCCAAGCGCGATTTCAAACGCCTGGCCAGCCTGTGCCGGTTCTCCGAAGACGCCATCAAGGGCGCGATGGCCGTGATTG
CGCGCCTGGACCCCAAGCCCGGCCGGCGCTTTGCCAATGTCGAGCGCAACCTGATCGTTCCCGACGTGATCGTCGTCAAG
TCCGGCCGGGGCTTCAAGGTCACGCTCAACAGCGACGTGATGCCCCGGCTGCGGGTCCACGACATCTACGCCAACGCCCT
CAAGCAGCACAGCGGCCGGGGCAGCAGCGGCCCGGGCGGCGGGCAGGCGCTGCAGCAGCGGCTGCAGGAAGCGCGCTGGT
TCATCAAGAACATCCAGCAGCGTTTCGACACCATCCTGCGGGTCAGCAACGCCATCGTGGAGCGGCAGAAAAACTTCTTC
ACCCACGGCGAACTCGCCATGCGGCCGCTGGTGCTGCGCGAGATCGCCGACGAGCTTGGCCTGCACGAATCGACCATTTC
GCGCGTGACCACGGCCAAGTACATGGGCACGCCGTTCGGCACCTTCGAGCTGAAGTATTTCTTTGGCTCCGGGCTGGGCA
CCGAGAGCGGCGGCAATGCGTCGAGCACCGCCGTGCGCGCGCTCATCAAGCAGTTCGTCGCCTCCGAAAGCCCCAAGAAG
CCGCTCAGCGACAGCCAGATTTCCGAGATGCTCAAGGAGCAGGGCATCGAATGCGCGCGCCGCACGGTGGCCAAATACCG
CGAGGCCCTGCGCATCGCACCGACCAATTTGCGCAAGGCGCTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-343)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(363-519)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.131

100

0.537

Multiple sequence alignment