Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL841_RS00815 Genome accession   NZ_CP157636
Coordinates   181823..183415 (+) Length   530 a.a.
NCBI ID   WP_018904016.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 4MFCol3.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 176823..188415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL841_RS00790 (ABL841_00790) argS 176881..178566 (+) 1686 WP_012745624.1 arginine--tRNA ligase -
  ABL841_RS00795 (ABL841_00795) - 178574..179307 (+) 734 Protein_158 SPOR domain-containing protein -
  ABL841_RS00800 (ABL841_00800) - 179453..180100 (+) 648 WP_018904013.1 thiol:disulfide interchange protein DsbA/DsbL -
  ABL841_RS00805 (ABL841_00805) lptA 180295..181014 (+) 720 WP_018904014.1 lipopolysaccharide transport periplasmic protein LptA -
  ABL841_RS00810 (ABL841_00810) lptB 181011..181805 (+) 795 WP_018904015.1 LPS export ABC transporter ATP-binding protein -
  ABL841_RS00815 (ABL841_00815) rpoN/rpoN1 181823..183415 (+) 1593 WP_018904016.1 RNA polymerase factor sigma-54 Machinery gene
  ABL841_RS00820 (ABL841_00820) - 183426..185030 (+) 1605 WP_018904017.1 phospholipase D family protein -
  ABL841_RS00825 (ABL841_00825) - 185041..185688 (-) 648 WP_018904018.1 LysE family translocator -
  ABL841_RS00830 (ABL841_00830) - 185707..186342 (-) 636 WP_012745632.1 YceI family protein -
  ABL841_RS00835 (ABL841_00835) - 186383..186964 (-) 582 WP_018904019.1 YceI family protein -
  ABL841_RS00840 (ABL841_00840) - 186961..187590 (-) 630 WP_018904020.1 cytochrome b -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58468.77 Da        Isoelectric Point: 4.7249

>NTDB_id=1007903 ABL841_RS00815 WP_018904016.1 181823..183415(+) (rpoN/rpoN1) [Variovorax paradoxus strain 4MFCol3.1]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAIDTPVPRDEAGEAAEGE
FSSAPASSATSTSSETASTGESEVPAADATEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDL
GECLCIQLRALATESETEEQALVRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQVISRLEPKPGRRFVDVER
NIVIPDVIVTKVGRGTNAKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1007903 ABL841_RS00815 WP_018904016.1 181823..183415(+) (rpoN/rpoN1) [Variovorax paradoxus strain 4MFCol3.1]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCACCTGGCGCTCACGCCCCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CCGCGCGCGAGGAATTCGGGCTCGACGCCATCGACACGCCGGTGCCGCGCGACGAGGCCGGTGAAGCCGCCGAGGGCGAA
TTCAGCTCGGCCCCGGCCAGCAGCGCGACCAGCACTTCGAGCGAAACCGCCTCCACGGGCGAGTCCGAGGTTCCCGCCGC
CGATGCCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCG
ATGCCCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAGTCGCTG
CAGTCCTTCCTGCACCGGCAGGCGCTGAGCCTGCGGCTGAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGAATC
GCTCAACGACGACGGCTACCTGGAGGATTCGCTGCCGGCGCTGGCCTCGGGCCTCGCGGGCGACGACAACGACCAGTTCG
ACGAGCTGGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGCCGGTGTGGGCGCGCGCGACCTG
GGCGAATGCCTTTGCATCCAGCTGCGCGCGCTCGCGACCGAAAGCGAGACCGAGGAACAGGCGCTGGTGCGCAAGACCGC
CATCGCCATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACG
AAGAGCTGGTGCGCTCGGCGCTGCAGGTCATCTCGCGCCTGGAGCCCAAGCCGGGCCGCCGTTTTGTCGACGTCGAGCGC
AACATCGTCATTCCCGACGTGATCGTCACCAAGGTCGGCCGCGGCACCAATGCCAAGTTCCGCGTCATGCTCAACCCCGA
AGTGATGCCGCGGCTGCGCGTGCACGACATCTATGCAGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCACGGCGAGCTCGCGATGCGACCCCTGGTGCTGCGCGAGATTGCCGACGA
ACTCGGCCTGCACGAATCGACCATCTCGCGCGTGACCACGGCCAAGTACATGTCCACGCCTTTCGGCACGGTCGAGCTCA
AGTACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTG
CGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCCCTGCGCATCGCGCCCGCCAACCTCCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.601

100

0.517

Multiple sequence alignment