Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL846_RS02315 Genome accession   NZ_CP157632
Coordinates   505776..507368 (+) Length   530 a.a.
NCBI ID   WP_418146656.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 295MFChir4.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 500776..512368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL846_RS02290 (ABL846_02290) argS 500830..502515 (+) 1686 WP_012745624.1 arginine--tRNA ligase -
  ABL846_RS02295 (ABL846_02295) - 502523..503260 (+) 738 WP_307592930.1 SPOR domain-containing protein -
  ABL846_RS02300 (ABL846_02300) - 503406..504053 (+) 648 WP_418146655.1 thiol:disulfide interchange protein DsbA/DsbL -
  ABL846_RS02305 (ABL846_02305) lptA 504248..504967 (+) 720 WP_018904014.1 lipopolysaccharide transport periplasmic protein LptA -
  ABL846_RS02310 (ABL846_02310) lptB 504964..505758 (+) 795 WP_412510239.1 LPS export ABC transporter ATP-binding protein -
  ABL846_RS02315 (ABL846_02315) rpoN/rpoN1 505776..507368 (+) 1593 WP_418146656.1 RNA polymerase factor sigma-54 Machinery gene
  ABL846_RS02320 (ABL846_02320) - 507379..508983 (+) 1605 WP_418146658.1 phospholipase D family protein -
  ABL846_RS02325 (ABL846_02325) - 508994..509641 (-) 648 WP_012745631.1 LysE family translocator -
  ABL846_RS02330 (ABL846_02330) - 509660..510295 (-) 636 WP_418146659.1 YceI family protein -
  ABL846_RS02335 (ABL846_02335) - 510336..510917 (-) 582 WP_418146660.1 YceI family protein -
  ABL846_RS02340 (ABL846_02340) - 510914..511543 (-) 630 WP_418146661.1 cytochrome b -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58542.85 Da        Isoelectric Point: 4.7249

>NTDB_id=1007869 ABL846_RS02315 WP_418146656.1 505776..507368(+) (rpoN/rpoN1) [Variovorax paradoxus strain 295MFChir4.1]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAIDTPVPRDEAGEAAEGE
FSSAPASSTTSTSSETTSTAESEVPAADATEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDL
GECLCIQLRALATESETEEQALVRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQVISRLEPKPGRRFVDVER
NIVIPDVIVTKVGRGTNAKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1007869 ABL846_RS02315 WP_418146656.1 505776..507368(+) (rpoN/rpoN1) [Variovorax paradoxus strain 295MFChir4.1]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCACCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CCGCGCGCGAGGAATTCGGGCTCGACGCCATCGACACGCCGGTGCCGCGCGACGAGGCCGGTGAAGCCGCCGAGGGCGAA
TTCAGCTCGGCCCCGGCCAGCAGCACGACCAGCACTTCGAGCGAAACCACCTCCACGGCCGAGTCCGAGGTTCCCGCCGC
CGACGCCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCG
ATGCCCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAATCGCTG
CAGTCCTTCCTGCACCGGCAGGCGCTGAGCCTGCGGCTGAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGAATC
GCTCAACGACGACGGCTACCTGGAGGATTCGCTGCCGGCGCTGGCCTCGGGCCTCGCGGGCGACGACAACGACCAGTTCG
ACGAGCTGGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGCCGGCGTGGGCGCGCGCGACCTG
GGCGAATGCCTTTGCATCCAGCTGCGCGCGCTCGCGACCGAAAGCGAGACCGAGGAACAGGCGCTGGTGCGCAAGACCGC
CATCGCGATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACG
AAGAGCTGGTGCGCTCGGCGCTGCAGGTCATCTCGCGCCTGGAGCCCAAGCCGGGCCGCCGCTTCGTCGACGTCGAGCGC
AACATCGTGATTCCCGACGTGATCGTCACCAAGGTCGGCCGCGGCACCAATGCCAAGTTCCGCGTCATGCTCAATCCCGA
GGTGATGCCGCGGCTGCGCGTGCACGACATCTATGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCACGGCGAGCTCGCGATGCGGCCCCTGGTGCTGCGCGAGATTGCCGACGA
ACTCGGCCTGCACGAATCGACCATCTCGCGCGTGACCACGGCCAAGTACATGTCCACGCCTTTCGGCACGGTCGAGCTCA
AGTACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCATCGAGCACCGCGGTGCGCGCGCTGATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTG
CGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCCCTGCGCATCGCGCCCGCCAACCTCCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.601

100

0.517

Multiple sequence alignment