Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL843_RS03000 Genome accession   NZ_CP157620
Coordinates   682034..683635 (+) Length   533 a.a.
NCBI ID   WP_418118917.1    Uniprot ID   -
Organism   Variovorax sp. 350MFTsu5.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677034..688635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL843_RS02975 (ABL843_02975) argS 677045..678730 (+) 1686 WP_418118914.1 arginine--tRNA ligase -
  ABL843_RS02980 (ABL843_02980) - 678742..679521 (+) 780 WP_418118915.1 SPOR domain-containing protein -
  ABL843_RS02985 (ABL843_02985) - 679697..680344 (+) 648 WP_126022201.1 thiol:disulfide interchange protein DsbA/DsbL -
  ABL843_RS02990 (ABL843_02990) lptA 680496..681221 (+) 726 WP_418118916.1 lipopolysaccharide transport periplasmic protein LptA -
  ABL843_RS02995 (ABL843_02995) lptB 681218..682012 (+) 795 WP_184640021.1 LPS export ABC transporter ATP-binding protein -
  ABL843_RS03000 (ABL843_03000) rpoN/rpoN1 682034..683635 (+) 1602 WP_418118917.1 RNA polymerase factor sigma-54 Machinery gene
  ABL843_RS03005 (ABL843_03005) - 683646..685250 (+) 1605 WP_418118918.1 phospholipase D family protein -
  ABL843_RS03010 (ABL843_03010) - 685261..685908 (-) 648 WP_418118919.1 LysE family translocator -
  ABL843_RS03015 (ABL843_03015) - 685925..686572 (-) 648 WP_184640015.1 YceI family protein -
  ABL843_RS03020 (ABL843_03020) - 686652..687257 (-) 606 WP_184640013.1 YceI family protein -
  ABL843_RS03025 (ABL843_03025) - 687254..687850 (-) 597 WP_418118920.1 cytochrome b -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 58452.42 Da        Isoelectric Point: 4.5560

>NTDB_id=1007757 ABL843_RS03000 WP_418118917.1 682034..683635(+) (rpoN/rpoN1) [Variovorax sp. 350MFTsu5.1]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAVREEFGLDTADAPVPRDDGGEGGEGE
SDFTPSAASSSTTSSSTESTAAADSDGPAAEVAEREPDWEGDGTVDMAPDDSEWGGDAPARQNNAGDDERADATELARSQ
ESLQSFLHRQALSLRLNESDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDEFDDLVHHFQVALGLLQSLEPAGVGA
RDLGECLSIQLRAMASDEETEEEELVRKTALAICKQPMELLARRDFKRLATLTRSSEELVRSALQVIARLEPKPGRSFVD
VERNVVIPDVIVTKTGRGTNVKFRVMLNPDVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRV
SNAIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRAL
IKQFVGSESVKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=1007757 ABL843_RS03000 WP_418118917.1 682034..683635(+) (rpoN/rpoN1) [Variovorax sp. 350MFTsu5.1]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCATCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCAGGCTGCTGCA
GCTCTCCACGCTCGAGCTGAGCCAGGAGGTGGAGCAGATGCTCGACGAGAACCCGTTCCTCGAGCGCACAGCCGAAGAAG
CGGTGCGCGAGGAATTCGGGCTCGACACAGCCGACGCGCCGGTCCCCCGCGACGACGGCGGTGAAGGCGGCGAAGGCGAG
AGCGACTTCACCCCCTCGGCCGCGAGCAGCAGCACCACCAGTTCGTCCACGGAAAGCACCGCGGCCGCCGACTCCGACGG
CCCCGCCGCCGAAGTCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAAT
GGGGCGGCGACGCGCCTGCGCGCCAGAACAACGCCGGCGACGACGAGCGCGCCGATGCCACCGAGCTCGCGCGCAGCCAG
GAGTCGCTGCAGTCCTTTTTGCACCGCCAGGCGCTGAGCCTGCGGCTCAACGAGAGCGACAGCGCCGCGCTGCGCTTTCT
CATCGAATCGCTCAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCGCTGGCCTCGGGCCTGGCCGGCGACGACAACG
ACGAGTTCGACGACCTCGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCCGCCGGCGTCGGCGCG
CGCGACCTCGGCGAGTGCCTGAGCATCCAGCTGCGTGCCATGGCCAGCGACGAGGAGACCGAGGAAGAAGAGCTGGTCCG
CAAGACCGCGCTGGCCATCTGCAAGCAGCCCATGGAGCTGCTCGCGCGACGCGACTTCAAGCGCCTGGCCACGCTCACCC
GCAGCAGCGAGGAGCTGGTGCGCTCGGCGCTGCAGGTCATCGCGCGGCTGGAGCCCAAGCCGGGCCGCAGCTTCGTCGAC
GTGGAACGCAACGTCGTCATTCCCGACGTGATCGTCACCAAGACCGGGCGCGGCACCAACGTGAAGTTCCGCGTCATGCT
CAACCCCGACGTGATGCCGCGGCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCC
AGGCGCTGTCGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTG
AGCAACGCCATCGTCGAACGGCAGAAGAGCTACTTCGTGCACGGCGAGCTCGCCATGCGCCCGCTGGTGCTGCGCGAGAT
CGCCGACGAGCTGGGCCTGCACGAGTCCACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGG
TCGAGCTCAAGTACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTC
ATCAAGCAGTTCGTCGGCTCGGAGAGCGTGAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTGAAGGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAGTACCGCGAAGCATTGCGCATCGCCCCTGCCAACCTGCGCAAGGCACTCT
AG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(154-361)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.434

100

0.525

Multiple sequence alignment