Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL849_RS03085 Genome accession   NZ_CP157618
Coordinates   736386..737984 (+) Length   532 a.a.
NCBI ID   WP_418130451.1    Uniprot ID   -
Organism   Variovorax sp. 375MFSha3.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 731386..742984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL849_RS03060 (ABL849_03060) argS 731406..733091 (+) 1686 WP_418118914.1 arginine--tRNA ligase -
  ABL849_RS03065 (ABL849_03065) - 733103..733879 (+) 777 WP_418130450.1 SPOR domain-containing protein -
  ABL849_RS03070 (ABL849_03070) - 734055..734702 (+) 648 WP_126022201.1 thiol:disulfide interchange protein DsbA/DsbL -
  ABL849_RS03075 (ABL849_03075) lptA 734854..735573 (+) 720 WP_126022200.1 lipopolysaccharide transport periplasmic protein LptA -
  ABL849_RS03080 (ABL849_03080) lptB 735570..736364 (+) 795 WP_184640021.1 LPS export ABC transporter ATP-binding protein -
  ABL849_RS03085 (ABL849_03085) rpoN/rpoN1 736386..737984 (+) 1599 WP_418130451.1 RNA polymerase factor sigma-54 Machinery gene
  ABL849_RS03090 (ABL849_03090) - 737995..739599 (+) 1605 WP_418132900.1 phospholipase D family protein -
  ABL849_RS03095 (ABL849_03095) - 739610..740257 (-) 648 WP_369659840.1 LysE family translocator -
  ABL849_RS03100 (ABL849_03100) - 740274..740921 (-) 648 WP_184640015.1 YceI family protein -
  ABL849_RS03105 (ABL849_03105) - 741001..741606 (-) 606 WP_184640013.1 YceI family protein -
  ABL849_RS03110 (ABL849_03110) - 741603..742199 (-) 597 WP_418133155.1 cytochrome b -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58381.34 Da        Isoelectric Point: 4.5537

>NTDB_id=1007739 ABL849_RS03085 WP_418130451.1 736386..737984(+) (rpoN/rpoN1) [Variovorax sp. 375MFSha3.1]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAVREEFGLDTADAPVPRDDGGEGEGES
DFTPSAASSSTTSSSTESTAAADSDGPAAEVAEREPDWEGDGTVDMAPDDSEWGGDAPARQNNAGDDERADATELARSQE
SLQSFLHRQALSLRLNESDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDEFDDLVHHFQVALGLLQSLEPAGVGAR
DLGECLSIQLRAMASDDETEEEELVRKTALAICKQPMELLARRDFKRLATLTRSSEELVRSALQVIARLEPKPGRSFVDV
ERNVVIPDVIVTKTGRGTNVKFRVMLNPDVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVS
NAIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALI
KQFVGSESVKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=1007739 ABL849_RS03085 WP_418130451.1 736386..737984(+) (rpoN/rpoN1) [Variovorax sp. 375MFSha3.1]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCATCTGGCGCTCACGCCGCAGCTGCAGCAGTCAATCAGGCTGCTGCA
GCTCTCCACGCTCGAGCTGAGCCAGGAGGTGGAGCAGATGCTCGACGAGAACCCGTTCCTCGAGCGCACAGCCGAAGAAG
CGGTGCGCGAGGAATTCGGGCTCGACACAGCCGACGCGCCGGTCCCCCGCGACGACGGCGGTGAAGGCGAAGGCGAGAGC
GACTTCACCCCCTCGGCCGCGAGCAGCAGCACCACCAGTTCGTCCACGGAAAGCACCGCGGCCGCCGACTCCGACGGCCC
CGCCGCCGAAGTCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGG
GCGGCGACGCGCCCGCGCGCCAGAACAACGCCGGCGACGACGAGCGCGCCGATGCCACCGAGCTCGCGCGCAGCCAGGAG
TCGCTGCAGTCCTTTTTGCACCGCCAGGCGCTGAGCCTGCGGCTCAACGAGAGCGACAGCGCCGCGCTGCGCTTTCTCAT
CGAATCGCTCAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCGCTGGCCTCGGGCCTGGCCGGTGACGACAACGACG
AGTTCGACGACCTCGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCCGCCGGCGTCGGCGCGCGC
GACCTCGGGGAGTGCCTGAGCATCCAGCTGCGCGCCATGGCGAGCGACGACGAGACCGAGGAAGAGGAGCTGGTCCGCAA
GACCGCGCTGGCCATCTGCAAGCAGCCCATGGAGCTGCTCGCGCGCCGCGACTTCAAGCGCCTGGCCACGCTCACCCGCA
GCAGCGAGGAGCTGGTGCGCTCGGCGCTGCAGGTCATCGCGCGGCTGGAGCCCAAGCCGGGCCGCAGCTTCGTCGACGTG
GAACGCAACGTCGTCATTCCCGACGTGATCGTCACCAAGACCGGGCGCGGCACCAACGTGAAGTTCCGCGTCATGCTCAA
CCCCGACGTGATGCCGCGGCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGG
CGCTGTCGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGC
AACGCCATCGTCGAACGGCAGAAGAGCTACTTCGTGCACGGCGAGCTCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGC
CGACGAGCTGGGCCTGCACGAGTCCACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGGTCG
AGCTCAAGTACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTCATC
AAGCAGTTCGTCGGCTCGGAGAGCGTGAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTGAAGGAGCAGGGCAT
CGAGTGCGCGCGCCGCACCGTCGCCAAGTACCGCGAAGCATTGCGCATCGCCCCTGCCAACCTGCGCAAGGCACTCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-530)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(153-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.72

100

0.528

Multiple sequence alignment