Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL847_RS16335 Genome accession   NZ_CP157616
Coordinates   3572240..3573889 (-) Length   549 a.a.
NCBI ID   WP_077001388.1    Uniprot ID   -
Organism   Variovorax sp. KK3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3567240..3578889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL847_RS16310 (ABL847_16285) - 3568129..3568419 (+) 291 WP_143684739.1 hypothetical protein -
  ABL847_RS16315 (ABL847_16290) - 3568642..3569253 (+) 612 WP_077001386.1 cytochrome b -
  ABL847_RS16320 (ABL847_16295) - 3569250..3569840 (+) 591 WP_077001387.1 YceI family protein -
  ABL847_RS16325 (ABL847_16300) - 3569866..3570540 (+) 675 WP_143684740.1 YceI family protein -
  ABL847_RS16330 (ABL847_16305) - 3570627..3572204 (-) 1578 WP_077001398.1 phospholipase D-like domain-containing protein -
  ABL847_RS16335 (ABL847_16310) rpoN/rpoN1 3572240..3573889 (-) 1650 WP_077001388.1 RNA polymerase factor sigma-54 Machinery gene
  ABL847_RS16340 (ABL847_16315) lptB 3573901..3574710 (-) 810 WP_077001389.1 LPS export ABC transporter ATP-binding protein -
  ABL847_RS16345 (ABL847_16320) lptA 3574707..3575417 (-) 711 WP_418127003.1 lipopolysaccharide transport periplasmic protein LptA -
  ABL847_RS16350 (ABL847_16325) - 3575534..3576178 (-) 645 WP_076999434.1 thiol:disulfide interchange protein DsbA/DsbL -
  ABL847_RS16355 (ABL847_16330) - 3576263..3576991 (-) 729 WP_076999435.1 SPOR domain-containing protein -
  ABL847_RS16360 (ABL847_16335) argS 3577006..3578679 (-) 1674 WP_076999436.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 549 a.a.        Molecular weight: 60254.99 Da        Isoelectric Point: 4.4622

>NTDB_id=1007722 ABL847_RS16335 WP_077001388.1 3572240..3573889(-) (rpoN/rpoN1) [Variovorax sp. KK3]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELGQEVEQMLDENPFLERAADEAPREEFGLEAADTPVRDDDRQSEAVEA
SEASDAFDASATESDYTPGASSSSSSESASASAGEGESATGTEGSDSPETEPGWEGDGTADMAPDDSEWGGDAPARNNSG
PGDEERPDATELARSQESLQSFLHRQALSLRLEDNDRAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQ
VALGLLQSLEPAGVGARSLAECLTIQLRAQQDEDDTDEEEQVRRTAMAICKQPMELLAKRDFKRLATLTRSNEDEVRSAL
QLIARLEPKPGRRFVDVERNIVIPDVIVTRIGQGGNAKFRVMLNPEVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEAR
WFIKNIQQRFDTILRVSNAIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPYGTVELKYFFGSAL
GTETGGNASSTAVRALIKQFVSAEDVKKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1650 bp        

>NTDB_id=1007722 ABL847_RS16335 WP_077001388.1 3572240..3573889(-) (rpoN/rpoN1) [Variovorax sp. KK3]
ATGAAGCAAGGGCTCTCGCTGCGCGTTTCGCAGCACCTCGCGCTGACGCCCCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAGCTGGGCCAGGAGGTCGAGCAGATGCTCGACGAAAACCCTTTCCTGGAACGCGCCGCCGACGAAG
CGCCGCGCGAGGAGTTCGGCCTGGAAGCCGCCGACACGCCGGTGCGCGACGACGACCGCCAGTCCGAAGCCGTAGAAGCC
TCGGAAGCCTCCGATGCCTTCGACGCCTCGGCAACGGAAAGCGATTACACGCCCGGCGCATCATCGTCCTCGTCCTCCGA
AAGCGCCTCGGCGTCGGCCGGCGAAGGCGAGTCGGCCACCGGCACCGAGGGCTCCGACAGCCCCGAGACCGAGCCCGGCT
GGGAAGGCGACGGCACGGCCGACATGGCGCCCGACGACAGCGAGTGGGGCGGCGACGCCCCCGCCCGCAACAACAGCGGC
CCTGGCGACGAGGAGCGCCCCGATGCCACCGAGCTCGCCCGCAGCCAGGAGTCGCTGCAGTCCTTCCTGCACCGCCAGGC
GCTGTCGCTGCGCCTCGAAGACAACGACCGCGCGGCCCTGCGCTTCCTGATCGAATCGCTCAACGACGACGGCTACCTTG
AAGATTCGCTGCCCGCGCTGGCCTCGGGCCTGGCCGGCGACGACAACGACCAGTTCGACGACCTGGTCCATCACTTCCAG
GTTGCGCTGGGCCTGCTGCAGAGCCTGGAGCCGGCCGGCGTCGGCGCGCGCAGCCTGGCCGAGTGCCTCACCATCCAGCT
GCGCGCGCAGCAGGACGAGGACGACACCGACGAGGAAGAGCAGGTGCGCCGCACCGCGATGGCCATCTGCAAGCAGCCGA
TGGAGCTGCTGGCCAAGCGCGACTTCAAGCGACTCGCCACGCTCACGCGCAGCAACGAGGACGAGGTGCGCTCCGCGCTG
CAGCTCATCGCCCGGCTCGAACCCAAGCCCGGGCGCCGCTTCGTCGACGTCGAGCGCAACATCGTCATCCCGGACGTCAT
CGTCACCCGCATCGGCCAGGGCGGCAATGCGAAGTTCCGCGTCATGCTCAATCCCGAGGTCATGCCGCGGCTGCGCGTGC
ACGACATCTATGCCGGCGCGCTGAAGGCGCACAAGGGCGAAGGCAGCCAGGCGCTGTCGCAGCGGCTGCAGGAGGCGCGC
TGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAACGCCATCGTCGAGCGGCAGAAGAGCTA
CTTCGTCCACGGCGAGCTGGCCATGCGGCCGCTGGTGCTGCGCGAGATCGCCGACGAACTCGGCCTGCACGAATCGACCA
TCAGCCGCGTGACGACCGCCAAGTACATGGCCACGCCCTACGGCACGGTCGAGCTCAAGTACTTCTTCGGCTCGGCGCTG
GGCACGGAGACCGGCGGCAATGCGTCCAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTCGTGAGCGCCGAGGATGTGAA
GAAGCCTTTGTCGGACAGCCAGGTGTCCGAGATGCTCAAGGAGCAGGGCATCGAATGCGCGCGCCGCACCGTGGCCAAGT
ACCGCGAGGCGCTGCGCATCGCGCCCGCGAACCTGCGCAAGGCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(391-547)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(170-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.454

100

0.506

Multiple sequence alignment