Detailed information NTG1007516

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABL390_RS17095
• Genome accession: NZ_CP157581
• Coordinates: 3718701..3719849 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NPA09B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3713701..3724849

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABL390_RS17075 (ABL390_17075)	-	3714439..3715623 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
ABL390_RS17080 (ABL390_17080)	-	3715620..3716993 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ABL390_RS17085 (ABL390_17085)	-	3717236..3717640 (-)	405	WP_003084560.1	NINE protein	-
ABL390_RS17090 (ABL390_17090)	czcD	3717794..3718693 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABL390_RS17095 (ABL390_17095)	pilU	3718701..3719849 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ABL390_RS17100 (ABL390_17100)	pilT	3720027..3721061 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABL390_RS17105 (ABL390_17105)	-	3721276..3721968 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABL390_RS17110 (ABL390_17110)	proC	3721980..3722801 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ABL390_RS17115 (ABL390_17115)	-	3722812..3723405 (+)	594	WP_003084544.1	YggT family protein	-
ABL390_RS17120 (ABL390_17120)	-	3723402..3723698 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1007516 ABL390_RS17095 WP_003100959.1 3718701..3719849(-) (pilU) [Pseudomonas aeruginosa strain NPA09B]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1007516 ABL390_RS17095 WP_003100959.1 3718701..3719849(-) (pilU) [Pseudomonas aeruginosa strain NPA09B]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382