Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BACILLUS_RS24050 Genome accession   NZ_CP157504
Coordinates   4574871..4575923 (-) Length   350 a.a.
NCBI ID   WP_047932633.1    Uniprot ID   -
Organism   Priestia megaterium strain FA-05     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4569871..4580923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BACILLUS_RS24010 (BACILLUS_004811) - 4570887..4571711 (+) 825 WP_016765555.1 YqhG family protein -
  BACILLUS_RS24015 (BACILLUS_004812) - 4571735..4571920 (-) 186 WP_013059170.1 YqzE family protein -
  BACILLUS_RS24020 (BACILLUS_004813) comGG 4571964..4572338 (-) 375 WP_016765556.1 competence type IV pilus minor pilin ComGG -
  BACILLUS_RS24025 (BACILLUS_004814) comGF 4572328..4572765 (-) 438 WP_016765557.1 competence type IV pilus minor pilin ComGF -
  BACILLUS_RS24030 (BACILLUS_004815) - 4572762..4573100 (-) 339 WP_016765558.1 type II secretion system protein -
  BACILLUS_RS24035 (BACILLUS_004816) comGD 4573084..4573521 (-) 438 WP_016765559.1 competence type IV pilus minor pilin ComGD -
  BACILLUS_RS24040 (BACILLUS_004817) comGC 4573508..4573819 (-) 312 WP_016765560.1 competence type IV pilus major pilin ComGC -
  BACILLUS_RS24045 (BACILLUS_004818) comGB 4573834..4574880 (-) 1047 WP_233335173.1 competence type IV pilus assembly protein ComGB -
  BACILLUS_RS24050 (BACILLUS_004819) comGA 4574871..4575923 (-) 1053 WP_047932633.1 competence type IV pilus ATPase ComGA Machinery gene
  BACILLUS_RS24055 (BACILLUS_004820) - 4576192..4576575 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BACILLUS_RS24060 (BACILLUS_004821) - 4576838..4577074 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  BACILLUS_RS24065 (BACILLUS_004822) - 4577112..4577744 (-) 633 WP_047932636.1 MBL fold metallo-hydrolase -
  BACILLUS_RS24070 (BACILLUS_004823) - 4577953..4578129 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  BACILLUS_RS24075 (BACILLUS_004824) - 4578165..4579067 (-) 903 WP_016765563.1 M14 family metallocarboxypeptidase -
  BACILLUS_RS24080 (BACILLUS_004825) - 4579179..4580147 (-) 969 WP_016765565.1 ROK family glucokinase -
  BACILLUS_RS24085 (BACILLUS_004826) - 4580150..4580356 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39670.99 Da        Isoelectric Point: 9.0036

>NTDB_id=1007348 BACILLUS_RS24050 WP_047932633.1 4574871..4575923(-) (comGA) [Priestia megaterium strain FA-05]
MTEIEQFATYVIRDAYECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAQIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1007348 BACILLUS_RS24050 WP_047932633.1 4574871..4575923(-) (comGA) [Priestia megaterium strain FA-05]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATATGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACATTAGAAGATCCTATTGAAAAACCGAGCGAAACCGTCCTTCAAGTACA
GGTAAATGAACGGGCAGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCCAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGCGTTAGCTTTCAAGAAATGGAACAGACCCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGAA
AACATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTAATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.857

100

0.589

Multiple sequence alignment