Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABLU28_RS03885 Genome accession   NZ_CP157495
Coordinates   747328..748689 (+) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain 2B-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 742328..753689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU28_RS03835 (ABLU28_03835) - 742620..743267 (+) 648 WP_058213293.1 adenylate kinase -
  ABLU28_RS03840 (ABLU28_03840) infA 743564..743782 (+) 219 WP_003130554.1 translation initiation factor IF-1 -
  ABLU28_RS03845 (ABLU28_03845) rpmJ 743836..743952 (+) 117 WP_001808836.1 50S ribosomal protein L36 -
  ABLU28_RS03850 (ABLU28_03850) rpsM 743970..744335 (+) 366 WP_003130555.1 30S ribosomal protein S13 -
  ABLU28_RS03855 (ABLU28_03855) rpsK 744356..744739 (+) 384 WP_010906297.1 30S ribosomal protein S11 -
  ABLU28_RS03860 (ABLU28_03860) - 744787..745725 (+) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  ABLU28_RS03865 (ABLU28_03865) rplQ 745744..746124 (+) 381 WP_003130558.1 50S ribosomal protein L17 -
  ABLU28_RS03875 (ABLU28_03875) - 746521..746640 (+) 120 WP_003130560.1 hypothetical protein -
  ABLU28_RS03880 (ABLU28_03880) - 746939..747166 (+) 228 WP_010906294.1 heavy-metal-associated domain-containing protein -
  ABLU28_RS03885 (ABLU28_03885) radA 747328..748689 (+) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  ABLU28_RS03890 (ABLU28_03890) - 748733..749419 (-) 687 WP_406834812.1 YoaK family protein -
  ABLU28_RS03895 (ABLU28_03895) - 749650..750735 (+) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  ABLU28_RS03900 (ABLU28_03900) - 750799..751428 (+) 630 WP_023188601.1 hypothetical protein -
  ABLU28_RS03905 (ABLU28_03905) - 751549..751911 (+) 363 WP_003129889.1 PadR family transcriptional regulator -
  ABLU28_RS03910 (ABLU28_03910) - 751898..752245 (+) 348 WP_010906292.1 DUF1048 domain-containing protein -
  ABLU28_RS03915 (ABLU28_03915) - 752232..752555 (+) 324 WP_003129893.1 DUF1048 domain-containing protein -
  ABLU28_RS03920 (ABLU28_03920) - 752647..753141 (-) 495 WP_003129897.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=1007187 ABLU28_RS03885 WP_003130563.1 747328..748689(+) (radA) [Lactococcus lactis strain 2B-1]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1007187 ABLU28_RS03885 WP_003130563.1 747328..748689(+) (radA) [Lactococcus lactis strain 2B-1]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGGCGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGTCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGCTCAACTAATGAAATTGGAATCTTTGAGATGCAGGG
GCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment