Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABKJ37_RS00485 Genome accession   NZ_CP157386
Coordinates   113638..115008 (+) Length   456 a.a.
NCBI ID   WP_010761995.1    Uniprot ID   R2SNS2
Organism   Enterococcus rotai strain CMTB-CA6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108638..120008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKJ37_RS00465 - 109719..110549 (+) 831 WP_348921415.1 HipA domain-containing protein -
  ABKJ37_RS00470 - 110552..111277 (+) 726 WP_348921416.1 HIRAN domain-containing protein -
  ABKJ37_RS00475 - 111558..112943 (+) 1386 WP_348921417.1 hypothetical protein -
  ABKJ37_RS00480 - 113055..113534 (+) 480 WP_208929742.1 dUTP diphosphatase -
  ABKJ37_RS00485 radA 113638..115008 (+) 1371 WP_010761995.1 DNA repair protein RadA Machinery gene
  ABKJ37_RS00490 - 115098..116240 (+) 1143 WP_348921418.1 PIN/TRAM domain-containing protein -
  ABKJ37_RS00495 ispD 116227..116949 (+) 723 WP_348921419.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ABKJ37_RS00500 ispF 116959..117432 (+) 474 WP_348921420.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ABKJ37_RS00505 gltX 117448..118905 (+) 1458 WP_348921421.1 glutamate--tRNA ligase -
  ABKJ37_RS00510 epsC 119170..119718 (+) 549 WP_348921422.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49964.64 Da        Isoelectric Point: 7.6809

>NTDB_id=1006552 ABKJ37_RS00485 WP_010761995.1 113638..115008(+) (radA) [Enterococcus rotai strain CMTB-CA6]
MAKKAKVQFECQTCGYISPKYLGRCPNCGQWNTMTEEIIQDTTDRRARVSLTGKKTQPQRLAEVVPKKEPRVKTKLVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAEIGGKVLYVSGEESAEQIKMRAERLSSIDTEFYLYAETDMNEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMREHGLEEVANPSQVFLEERLADATGSAIVVAMEGTRPILVEVQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLAVAVSIASSYKENGTKPTECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKIYLPKNNLGGWTPPEGIEIVGVATIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1006552 ABKJ37_RS00485 WP_010761995.1 113638..115008(+) (radA) [Enterococcus rotai strain CMTB-CA6]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAACGTGCGGGTATATTTCCCCCAAATATTTAGGACGCTGTCCAAA
TTGCGGACAGTGGAACACAATGACAGAAGAAATCATTCAAGATACGACAGATCGTCGTGCTAGAGTTAGTTTAACAGGTA
AAAAGACACAGCCTCAGCGCTTAGCTGAAGTGGTTCCTAAGAAAGAACCAAGAGTGAAAACCAAACTCGTAGAGCTGAAC
CGTGTGTTAGGCGGCGGTGTCGTTCCTGGATCACTTGTTTTAATCGGTGGAGATCCTGGAATCGGTAAGTCAACGTTACT
GCTACAAGTGTCGCAACAACTTGCTGAAATTGGCGGCAAAGTCTTATATGTTTCCGGAGAAGAGAGCGCCGAACAAATCA
AAATGCGGGCAGAACGTTTAAGTTCGATCGATACGGAATTTTACTTATACGCTGAAACAGATATGAATGAAATCTCTCGT
GCAATCGAGAAATTAGAACCAGATTATGTGATCATTGACTCTATTCAGACGATGACGCAGCCAGATGTAACGAGTGTGGC
TGGTAGTGTGAGCCAAGTCCGTGAGACAACCGCTGAATTATTGAAAATCGCCAAAACCAATGGTATTGCGATTTTTATCG
TCGGTCACGTGACCAAAGAAGGTTCCATTGCAGGTCCTCGTATGCTTGAGCATATGGTAGATACAGTTTTATATTTTGAA
GGTGATAAACATCATACGTTCAGAATCTTAAGAGCGGTCAAAAATCGTTTTGGGTCGACTAATGAAATTGGTATTTTTGA
AATGCGAGAACATGGACTGGAAGAAGTTGCCAATCCATCGCAAGTCTTTTTAGAGGAACGACTGGCGGATGCCACAGGAT
CAGCAATCGTCGTAGCTATGGAAGGAACGCGGCCAATTTTGGTTGAGGTACAGGCTTTAGTTACACCAACTATGTTTGGA
AATGCTAAACGAACAACCACAGGGCTAGATTTCAATCGAGTTTCATTGATCATGGCAGTTCTAGAAAAGCGAGCTGGCTT
ACTCTTACAAAATCAAGATGCCTATTTGAAAGCAGCAGGCGGTGTCAAAATCAATGAACCAGCAATCGATTTGGCTGTAG
CGGTCAGTATTGCTTCTAGTTATAAAGAAAATGGGACCAAACCAACAGAATGTTTTATTGGTGAAATCGGGTTAACTGGC
GAAATACGTCGTGTGAACAGTATTGAACAACGAGTACGTGAAGCGCAAAAGCTAGGGTTCACCAAGATTTATTTGCCGAA
AAACAATTTAGGTGGTTGGACGCCACCAGAAGGAATCGAGATTGTCGGCGTTGCTACAATTGGAGAAACCTTAAGAAAAG
TATTCAAGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(70-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R2SNS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.725

99.781

0.746
  radA Streptococcus mitis SK321

74.725

99.781

0.746
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

66.075

98.904

0.654

Multiple sequence alignment