Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QUD86_RS05860 Genome accession   NZ_AP027274
Coordinates   1155756..1157120 (-) Length   454 a.a.
NCBI ID   WP_286295836.1    Uniprot ID   -
Organism   Polynucleobacter sp. TUM22923     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1150756..1162120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD86_RS05845 (TUM22923_11290) dapD 1152829..1153656 (+) 828 WP_100378805.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  QUD86_RS05850 (TUM22923_11300) dapE 1153672..1154823 (+) 1152 WP_286295832.1 succinyl-diaminopimelate desuccinylase -
  QUD86_RS05855 (TUM22923_11310) prmB 1154844..1155740 (+) 897 WP_286295835.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  QUD86_RS05860 (TUM22923_11320) radA 1155756..1157120 (-) 1365 WP_286295836.1 DNA repair protein RadA Machinery gene
  QUD86_RS05865 (TUM22923_11330) - 1157125..1157634 (-) 510 WP_286295838.1 hypothetical protein -
  QUD86_RS05870 (TUM22923_11340) - 1157631..1159472 (-) 1842 WP_286298689.1 potassium transporter Kup -
  QUD86_RS05880 (TUM22923_11350) - 1160008..1160436 (+) 429 WP_286295840.1 hypothetical protein -
  QUD86_RS05885 (TUM22923_11360) - 1160552..1161052 (-) 501 WP_286295842.1 hypothetical protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48146.95 Da        Isoelectric Point: 7.1451

>NTDB_id=100550 QUD86_RS05860 WP_286295836.1 1155756..1157120(-) (radA) [Polynucleobacter sp. TUM22923]
MAKIKTVYICQSCGGTSAKWQGQCPACQEWNTLEEGVPEVSSNSRFQGLAQSLPRQKLSAINAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLIGGDPGIGKSTLLLQALAEMSAMGMNVLYSSGEESAAQIALRAKRIALNAPHLEVLAEIQLEKLLSIMD
TVKPQVLVVDSIQTLYSDALSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVANPSAIFLSQHTEMVPGACVLVTQEGSRPLLVEIQALVDTAHIPNPR
RLAVGLDQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNKALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTIAIIPKANMPKAKIAGLTVIPVERIDQAISAAAELT

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=100550 QUD86_RS05860 WP_286295836.1 1155756..1157120(-) (radA) [Polynucleobacter sp. TUM22923]
TTGGCCAAAATTAAAACCGTTTATATCTGTCAGTCTTGTGGCGGTACTTCTGCAAAGTGGCAAGGACAGTGTCCAGCTTG
TCAGGAGTGGAACACCCTCGAAGAGGGCGTGCCTGAAGTAAGTTCAAATTCTCGGTTTCAGGGCTTGGCGCAATCTTTGC
CCCGTCAAAAACTGTCGGCTATTAATGCTGAGGATCTGCCGCGTTTTAGCACCGGTGTCGAAGAGTTTGACCGAGTACTT
GGTGGAGGGTTAGTGCCCGGCGGTGTTGTTCTCATCGGTGGTGACCCTGGCATTGGTAAGTCAACATTGTTATTGCAAGC
CCTAGCCGAGATGAGTGCTATGGGCATGAATGTTCTTTACAGCAGTGGCGAAGAATCCGCCGCCCAAATTGCTTTGCGGG
CAAAGCGTATCGCTTTAAATGCACCGCATTTAGAAGTGTTGGCAGAGATTCAGCTGGAGAAACTGTTATCTATTATGGAT
ACCGTGAAGCCGCAGGTATTGGTAGTCGATTCCATACAGACTTTATATTCTGATGCCTTGAGTTCCGCACCAGGTTCCGT
AGCGCAAGTTCGTGAGTGTGCCGCACAATTAACGAGAGCCGCTAAATCTAGTGGTATTTGCGTATTGATGGTGGGGCACG
TTACTAAAGATGGTCATCTTGCGGGCCCTAGAGTGCTCGAGCATATTGTTGATACAGTGTTGTATTTTGAAGGTGATACC
CATTCCTCTTTTAGGTTGGTACGTTCGATTAAAAATCGTTTCGGCGCGGTCAATGAGTTAGGTGTTTTTGCGATGACTGA
GAAGGGCTTACGCGGGGTTGCTAATCCATCAGCTATTTTCTTGTCGCAGCATACTGAGATGGTGCCTGGTGCCTGCGTAT
TAGTGACACAAGAGGGTAGTCGTCCGCTGTTGGTGGAGATTCAGGCGCTGGTAGATACTGCGCATATACCGAACCCTAGA
AGGTTGGCAGTTGGTTTGGATCAAGCGCGCTTAGCAATGCTCTTGGCGGTACTACATCGCCACGCTGGTGTTGCTTGTTT
TGATCAGGATGTATTCTTAAACGCGGTAGGTGGGGTTAAGATCTCAGAGCCTGCGGCTGACTTAGCGGTGCTGCTTGCCA
TTCAATCATCGATTCGCAATAAGGCACTTCCGAAAGAGCTGATTGTGTTTGGCGAAGTAGGCTTAGCCGGCGAGATTCGT
CCATGTCCTAGGGGTCAAGAGCGCTTAAAAGAGGCCGCAAAACTAGGCTTCACAATTGCCATTATTCCGAAGGCCAATAT
GCCTAAGGCAAAAATCGCTGGCTTAACAGTCATTCCAGTTGAGCGCATCGATCAGGCGATTTCTGCGGCGGCAGAGCTCA
CCTAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(359-450)

AAA domain (AAA_25)

(79-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.231

100

0.493
  radA Streptococcus mitis NCTC 12261

46.861

98.238

0.46
  radA Streptococcus pneumoniae Rx1

46.861

98.238

0.46
  radA Streptococcus pneumoniae D39

46.861

98.238

0.46
  radA Streptococcus pneumoniae R6

46.861

98.238

0.46
  radA Streptococcus pneumoniae TIGR4

46.861

98.238

0.46
  radA Streptococcus mitis SK321

47.552

94.493

0.449

Multiple sequence alignment