Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   E0F34_RS03065 Genome accession   NZ_LR536843
Coordinates   562244..564466 (+) Length   740 a.a.
NCBI ID   WP_001855317.1    Uniprot ID   A0A0H2UP28
Organism   Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 557244..569466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F34_RS03035 (SAMEA104035599_00579) - 557598..558788 (-) 1191 WP_000843701.1 ferric reductase-like transmembrane domain-containing protein -
  E0F34_RS03045 (SAMEA104035599_00580) - 559046..559705 (-) 660 WP_000443837.1 amino acid ABC transporter permease -
  E0F34_RS03050 (SAMEA104035599_00581) - 559715..560392 (-) 678 WP_000131161.1 amino acid ABC transporter permease -
  E0F34_RS03055 (SAMEA104035599_00582) - 560405..561169 (-) 765 WP_054422985.1 transporter substrate-binding domain-containing protein -
  E0F34_RS03060 (SAMEA104035599_00583) - 561209..561967 (-) 759 WP_001229583.1 amino acid ABC transporter ATP-binding protein -
  E0F34_RS03065 (SAMEA104035599_00584) recJ 562244..564466 (+) 2223 WP_001855317.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  E0F34_RS03070 (SAMEA104035599_00585) - 564641..566302 (+) 1662 WP_000065637.1 ribonuclease J -
  E0F34_RS03075 (SAMEA104035599_00586) - 566371..567150 (+) 780 WP_000290025.1 alpha/beta hydrolase -
  E0F34_RS03080 (SAMEA104035599_00587) murM 567262..568482 (+) 1221 WP_061649915.1 peptidoglycan bridge formation alanyltransferase MurM -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 82479.98 Da        Isoelectric Point: 4.3606

>NTDB_id=1004447 E0F34_RS03065 WP_001855317.1 562244..564466(+) (recJ) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEE
GENILVYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQVELLDLVAIGTIADMVSLTDENR
ILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKN
EERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDP
HRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKP
IFYIKNFQVESARTMGAGNAHLKLKISKGEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVE
GVQLFNIRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKNDIDKAYYLTGYGTRDQ
FAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKD
QEMMALGTVQEIYDFLMEKE

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=1004447 E0F34_RS03065 WP_001855317.1 562244..564466(+) (recJ) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
TTGATAACACCTACTTATGAATGGCAGTTTGCCCTGCAGGTAGAAGATGCGGATTTTACAAAGATAGCCAAGAAGGCTGG
ACTGGGTCCTGAGGTGGCTCGGTTATTGTTTGAGAGAGGGATTCAGAACCAAGAAAGTCTGAAGAAGTTTTTAGAACCTT
CCTTGGAGGACTTACATGATGCTTATCTGCTCCATGATATGGACAAGGCAGTGGAGCGGATTCGTCAGGCTATTGAAGAA
GGGGAAAATATTCTTGTTTATGGAGACTATGATGCGGATGGCATGACTTCGGCTTCTATTGTGAAGGAAAGTTTGGAACA
ACTTGGTGCTGAGTGCCGAGTTTACCTGCCAAATCGTTTTACCGATGGCTATGGCCCTAATGCTAGTGTTTATAAATACT
TTATCGAGCAAGAAGGGATTTCCTTGATTGTGACGGTGGACAATGGGGTTGCTGGTCATGAGGCTATTGCATTGGCTCAG
TCTATGGGAGTAGATGTCATTGTGACAGACCATCATTCCATGCCTGAAACCCTGCCAGATGCTTATGCTATTGTCCATCC
TGAACATCCAGATGCGGATTATCCTTTTAAATATTTGGCTGGTTGTGGAGTTGCTTTCAAGTTGGCTTGTGCCCTGTTAG
AAGAAGTGCAAGTGGAATTGCTTGATTTGGTCGCTATTGGAACTATTGCAGATATGGTGAGTCTGACGGATGAAAATCGT
ATCTTAGTTCAATATGGTCTGGAAATGTTGGGTCATACCCAGCGCATTGGTCTGCAAGAAATGCTGGACATGGCTGGGAT
TGCTGCCAACGAAGTAACAGAAGAAACGGTTGGTTTCCAGATTGCTCCTCGTTTGAATGCCTTGGGTCGCTTGGATGATC
CCAATCCTGCCATTGATTTGTTGACTGGATTTGATGATGAGGAAGCGCATGAGATTGCCCTTATGATTCACCAGAAAAAC
GAAGAGCGCAAGGAAATCGTTCAGTCTATCTATGAAGAAGCCAAGACCATGGTGGATCCTGAGAAGAAGGTTCAGGTCTT
GGCCAAGGAAGGCTGGAATCCTGGGGTTCTAGGAATCGTGGCTGGTCGTTTATTGGAAGAATTGGGACAGACAGTCATTG
TTCTTAATATAGAAGACGGTCGTGCCAAGGGCAGTGCTCGTAGTGTGGAAGCGGTCGATATTTTTGAAGCTCTGGATCCC
CATCGAGACCTCTTCATCGCCTTTGGAGGTCATGCAGGTGCAGCGGGTATGACGCTGGAAGTTGAGCAACTCTCAGATTT
ATCTCAGGTTTTGGAAGATTATGTTCGTGAAAAAGGTGCAGATGCTGGTGGCAAGAATAAGTTAAACCTAGATGAAGAGT
TGGATTTGGAGGCACTTAGCTTGGAAACGGTCAAAAGTTTTGAACGTTTAGCTCCTTTTGGAATGGATAATCAGAAACCT
ATTTTTTATATCAAGAATTTTCAGGTCGAAAGTGCTCGTACTATGGGGGCAGGTAATGCCCATCTAAAGCTGAAAATTTC
CAAGGGTGAGGCGAGTTTTGAAGTGGTAGCCTTTGGTCAAGGCAGATGGGCGACAGAGTTTTCTCAAACCAAGAATCTAG
AGTTAGCGGTTAAATTGTCTGTCAACCAATGGAATGGCCAAACTGCCCTCCAGTTGATGATGGTGGATGCGCGAGTGGAA
GGTGTTCAACTTTTTAACATTCGTGGAAAAAATGCAGTCTTGCCAGAAGGTGTTCCAGTCTTGGATTTTCCTGGAGAACT
GCCAAATCTTGCGGCTAGTGAAGCTGTTGTCGTAAAAAACATTCCAGAGGATATTACTCAGCTGAAGACCATTTTTCAGG
AACAGCATTTCTCTGCTGTCTATTTCAAAAATGATATTGACAAGGCTTATTATCTGACAGGTTATGGGACTAGAGATCAG
TTTGCCAAATTGTACAAGACTATTTACCAGTTCCCAGAGTTTGATATTCGCTACAAGCTGAAAGATTTGGCTGCATATCT
TAATATTCAACAAATCTTGCTGGTCAAGATGATTCAAGTATTTGAAGAACTAGGCTTTGTGACGATAAAAGATGGTGTGA
TGACAGTCAATAAAGAGGCGCCAAAGCGGGAGATAGGAGAAAGTCAAATTTACCAAAATCTCAAACAAACCGTTAAAGAC
CAAGAAATGATGGCGCTGGGTACGGTGCAAGAAATTTATGATTTTTTGATGGAAAAAGAGTAG

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(593-735)

RecJ OB domain (RecJ_OB)

(451-556)

DHHA1 domain (DHHA1)

(317-434)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2UP28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

99.865

100

0.999
  recJ Bacillus subtilis subsp. subtilis str. 168

36.917

100

0.385

Multiple sequence alignment