Detailed information NTG1004113

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: E0F40_RS08290
• Genome accession: NZ_LR216065
• Coordinates: 1569488..1571884 (-)
• Length: 798 a.a.
• NCBI ID: WP_001152092.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1564488..1576884

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F40_RS08270 (SAMEA3714487_01574)	stkP	1564503..1566482 (-)	1980	WP_000614540.1	serine/threonine-protein kinase StkP	Regulator
E0F40_RS08275 (SAMEA3714487_01575)	-	1566479..1567219 (-)	741	WP_000406251.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
E0F40_RS08280 (SAMEA3714487_01576)	rsmB	1567234..1568547 (-)	1314	WP_001813599.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
E0F40_RS08285 (SAMEA3714487_01577)	fmt	1568540..1569475 (-)	936	WP_000163703.1	methionyl-tRNA formyltransferase	-
E0F40_RS08290 (SAMEA3714487_01578)	priA	1569488..1571884 (-)	2397	WP_001152092.1	primosomal protein N'	Machinery gene
E0F40_RS08295 (SAMEA3714487_01579)	rpoZ	1571950..1572264 (-)	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
E0F40_RS08300 (SAMEA3714487_01580)	gmk	1572289..1572915 (-)	627	WP_000775037.1	guanylate kinase	-
E0F40_RS08305 (SAMEA3714487_01581)	-	1573045..1574658 (-)	1614	WP_000404940.1	ribonuclease Y	-
E0F40_RS08310 (SAMEA3714487_01582)	-	1574849..1575103 (-)	255	WP_000924721.1	Txe/YoeB family addiction module toxin	-
E0F40_RS08315 (SAMEA3714487_01583)	-	1575107..1575361 (-)	255	WP_000388572.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E0F40_RS08320 (SAMEA3714487_01584)	-	1575599..1576696 (-)	1098	WP_000156330.1	nucleotidyltransferase	-

Sequence

Protein

• Length: 798 a.a.
• Molecular weight: 90027.77 Da
• Isoelectric Point: 6.1573

>NTDB_id=1004113 E0F40_RS08290 WP_001152092.1 1569488..1571884(-) (priA) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYKQDSNPRYH
ARDVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Nucleotide

• Length: 2397 bp

>NTDB_id=1004113 E0F40_RS08290 WP_001152092.1 1569488..1571884(-) (priA) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGTTACAGGATTCCGGAGGAATT
TGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCTTTTGGTAAGGGGAATCGCCTGATTCAAGGGATTGTTCTTG
GTTTGGAGTCCCAATCAGATGGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAAATTTCTATCCTCAAGGCCAT
GCTTCCAGGATTTCTGAATTCTAGCTATGACAAGATTCTCTATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTGCGCC
TGTTTGGTTCAGAAGATTCGCTAGCCTTTTCTTCACTAGACCTTGCCAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
GGCCTGCTTGGTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGGTATGAGGTTGACCATGCTCA
ATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAGTTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCG
CTTCCTTGGCTAGCTTGTTAGAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
CAAAAGGAAGTTCAACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTGGAGTTAAATCCAGAACAAAG
ACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCTCAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGA
AGACCGAGGTTTACTTGCAGATTATCCAAGGTGCCCTGGATAAGGGCAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTTCACTCAGGATTATCCAATGG
TGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGATGCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTC
CTCTGAAAAATCTGGGTGTCATGATTATTGATGAAGAGCATGAAGTGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
GCCAGAGATGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCTGCAACGCCGAGCTTAGAGAG
CCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGTCTAACCCAACGTGCTAATCCTTTGGCTACAATCCCTGAGG
TTCAAGTGATTGACTTTCGAGACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGCTTTGTCATGTGTCGGGAGTG
TGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATT
GTGGTTTCTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTTTGAGGATGGATGTGGATACGACTCGAAAGAAAGG
CAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTT
TGGATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTA
CAATTCGCAGCACTATGCTATTCGATTTGCCAAGGATCAAGATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGAC
GACAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGC
CCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAATACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGG
TCTTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (700-794)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (513-538)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-109)

• Type III restriction enzyme, res subunit (ResIII) (260-424)

• Helicase conserved C-terminal domain (Helicase_C) (572-656)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	45.936	100	0.467