Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAEY33_RS00575 Genome accession   NZ_CP157082
Coordinates   106954..108342 (+) Length   462 a.a.
NCBI ID   WP_076372905.1    Uniprot ID   A0A9W4PDJ3
Organism   Peribacillus simplex strain IMGN11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101954..113342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEY33_RS00555 (AAEY33_00555) - 102069..102530 (+) 462 WP_098373579.1 CtsR family transcriptional regulator -
  AAEY33_RS00560 (AAEY33_00560) - 102552..103103 (+) 552 WP_098373578.1 UvrB/UvrC motif-containing protein -
  AAEY33_RS00565 (AAEY33_00565) - 103100..104176 (+) 1077 WP_144529692.1 protein arginine kinase -
  AAEY33_RS00570 (AAEY33_00570) clpC 104307..106748 (+) 2442 WP_098373576.1 ATP-dependent protease ATP-binding subunit ClpC -
  AAEY33_RS00575 (AAEY33_00575) radA 106954..108342 (+) 1389 WP_076372905.1 DNA repair protein RadA Machinery gene
  AAEY33_RS00580 (AAEY33_00580) disA 108345..109418 (+) 1074 WP_076372903.1 DNA integrity scanning diadenylate cyclase DisA -
  AAEY33_RS00585 (AAEY33_00585) - 109644..110741 (+) 1098 WP_076372901.1 PIN/TRAM domain-containing protein -
  AAEY33_RS00590 (AAEY33_00590) ispD 110763..111449 (+) 687 WP_347940985.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AAEY33_RS00595 (AAEY33_00595) ispF 111464..111943 (+) 480 WP_134784433.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50175.81 Da        Isoelectric Point: 8.2345

>NTDB_id=1003995 AAEY33_RS00575 WP_076372905.1 106954..108342(+) (radA) [Peribacillus simplex strain IMGN11]
MAVKKKTKFMCQSCGYESPKWMGKCPGCGEWNKMVEETEIVKPARKGAFTNSEVRGSGEREKAAPITTIQSEKEPRIKTD
LMELNRALGGGIVQGSLVLIGGDPGIGKSTLLLQVSSQLAHKQKKVLYISGEESVKQTKLRADRLGTMSENLFVYAETDM
DYIQQAITDVKPDLVIIDSIQTVYHSEVTSAPGSVSQVRECTASLMRIAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDT
VLYFEGERHHTYRIIRAVKNRFGSTNEMGIFEMKEHGLEEVANPSEIFLEERSQGASGSTVVASMEGTRPVLVEIQALIS
PTSFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAISIASSFRDKPTNPADCIIGE
VGLTGEVRRVSRIEQRVQEAAKLGFERVIIPANNIGGWTAPKGIKIVGVSSVSEALKQSLGG

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=1003995 AAEY33_RS00575 WP_076372905.1 106954..108342(+) (radA) [Peribacillus simplex strain IMGN11]
ATGGCTGTAAAGAAGAAAACAAAATTTATGTGTCAGTCTTGTGGATATGAGTCACCGAAATGGATGGGGAAATGCCCAGG
ATGCGGTGAATGGAACAAAATGGTCGAGGAAACCGAAATCGTTAAACCTGCTAGAAAAGGCGCTTTTACCAATTCAGAGG
TTAGAGGTTCCGGGGAACGGGAGAAGGCAGCACCCATAACGACTATTCAATCAGAAAAAGAACCCCGGATTAAGACGGAT
TTAATGGAATTGAATCGTGCTCTTGGGGGCGGGATCGTACAGGGATCACTTGTATTGATCGGAGGGGACCCGGGTATCGG
TAAGTCCACCCTGCTTTTACAGGTATCATCCCAATTGGCGCATAAACAGAAAAAAGTGCTTTATATTTCTGGTGAAGAAT
CAGTCAAGCAGACCAAATTGAGAGCGGATCGGCTTGGGACCATGTCGGAAAATCTATTCGTTTATGCTGAAACCGATATG
GATTATATCCAACAGGCAATTACGGATGTAAAACCGGATTTGGTCATTATTGACTCCATTCAAACGGTATACCATTCGGA
GGTTACATCTGCCCCAGGAAGCGTTTCACAAGTGAGGGAGTGCACAGCCTCACTCATGCGCATTGCTAAAACGAACGGAA
TCGCGATTTTTATCGTCGGACACGTTACAAAGGAAGGGGCCATCGCGGGGCCACGGCTGCTTGAGCACATGGTTGATACA
GTATTATATTTTGAAGGTGAAAGACACCATACATATCGAATTATACGTGCGGTTAAAAATCGCTTTGGTTCGACGAATGA
GATGGGTATTTTTGAAATGAAAGAACATGGATTGGAAGAGGTCGCGAATCCATCGGAAATTTTCCTTGAAGAACGGTCGC
AGGGAGCCTCGGGTTCCACAGTCGTCGCATCCATGGAGGGCACAAGGCCGGTGCTAGTCGAAATTCAAGCATTGATTTCA
CCTACGAGTTTTGGCAATCCGAGGCGAATGGCCACGGGAATCGACCACAATCGAGTTTCGCTTTTAATGGCTGTTCTGGA
AAAGAGAGTGGGCTTGCTTCTTCAAAACCAAGATGCGTATTTAAAGGTTGCCGGTGGTGTCAAGCTGGATGAACCTGCAA
TTGACCTCGCTGTCGCAATAAGCATTGCTTCAAGCTTCCGTGATAAACCAACAAACCCTGCCGATTGCATCATTGGTGAA
GTGGGGCTGACTGGTGAGGTAAGAAGGGTGTCAAGAATTGAACAAAGGGTGCAGGAAGCAGCAAAATTAGGGTTTGAGCG
GGTTATCATACCGGCTAATAATATAGGGGGATGGACCGCGCCGAAGGGTATTAAGATCGTTGGTGTTTCATCGGTTTCGG
AGGCTCTTAAACAATCGTTAGGGGGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-226)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(369-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.174

99.567

0.768
  radA Streptococcus mitis NCTC 12261

62.418

98.485

0.615
  radA Streptococcus pneumoniae Rx1

61.978

98.485

0.61
  radA Streptococcus pneumoniae D39

61.978

98.485

0.61
  radA Streptococcus pneumoniae R6

61.978

98.485

0.61
  radA Streptococcus pneumoniae TIGR4

61.978

98.485

0.61
  radA Streptococcus mitis SK321

61.758

98.485

0.608

Multiple sequence alignment