Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABG777_RS00540 Genome accession   NZ_CP157073
Coordinates   118940..120307 (+) Length   455 a.a.
NCBI ID   WP_077281988.1    Uniprot ID   A0A5B8TEG9
Organism   Leuconostoc lactis strain APC3969     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 113940..125307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABG777_RS00510 - 114263..115687 (-) 1425 WP_010002512.1 aspartate ammonia-lyase -
  ABG777_RS00515 rpiA 115850..116539 (-) 690 WP_010000960.1 ribose-5-phosphate isomerase RpiA -
  ABG777_RS00520 - 116536..116817 (-) 282 WP_010002509.1 GNAT family N-acetyltransferase -
  ABG777_RS00525 - 117043..117582 (+) 540 WP_010000962.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  ABG777_RS00530 - 117590..118054 (+) 465 WP_010000963.1 NUDIX hydrolase -
  ABG777_RS00535 - 118072..118953 (+) 882 WP_010002507.1 metallophosphoesterase family protein -
  ABG777_RS00540 radA 118940..120307 (+) 1368 WP_077281988.1 DNA repair protein RadA Machinery gene
  ABG777_RS00545 - 120318..120641 (+) 324 WP_010002503.1 hypothetical protein -
  ABG777_RS00550 gltX 120817..122313 (+) 1497 WP_189428870.1 glutamate--tRNA ligase -
  ABG777_RS00555 - 122450..123355 (+) 906 WP_189428873.1 NADP-dependent oxidoreductase -
  ABG777_RS00560 adhP 123518..124573 (+) 1056 WP_029510101.1 alcohol dehydrogenase AdhP -
  ABG777_RS00565 - 124735..125274 (+) 540 WP_019040920.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49227.40 Da        Isoelectric Point: 5.2659

>NTDB_id=1003971 ABG777_RS00540 WP_077281988.1 118940..120307(+) (radA) [Leuconostoc lactis strain APC3969]
MAKVKTQFICSNCGFTSARYLGRCSNCGEWGTFVEEKIMPETNDRKSRVSLDGRTAKVEKINEVTSEETPRVATNLKELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGQLAHEGRVLYVTGEESATQVKLRADRLGVGNDEFYLYPETDMTAIKKQ
IESLQPDFVVIDSVQTMQEPDVTSAIGSVSQIREVTADLLQIAKTNNISIFIVGHVTKDGAIAGPKILEHMVDTVLYFEG
DSNYKYRILRAVKNRFGATNELGIFEMRDGGLIEVANPSEIFLEERLAGATGSAIVVALEGSRPILVELQALVTPTVFGN
AQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYHDKESRPSDVFVGEIGLTGE
IRSVADIESRLKEARKLGFKRAIVPKNNLNGVTLPEGIQVIGVATLREALQLALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1003971 ABG777_RS00540 WP_077281988.1 118940..120307(+) (radA) [Leuconostoc lactis strain APC3969]
ATCGCAAAAGTTAAAACACAATTTATTTGCTCAAACTGTGGTTTCACGTCAGCGCGCTATCTGGGTCGCTGCTCAAACTG
TGGTGAATGGGGCACATTTGTCGAAGAAAAAATTATGCCAGAGACCAACGACCGGAAAAGTCGGGTGAGCCTAGACGGTC
GTACGGCTAAGGTTGAAAAGATTAATGAGGTCACATCAGAGGAAACACCGCGTGTTGCCACGAACCTTAAAGAACTGAAC
CGCGTGTTAGGCGGTGGGGTGGTTCCAGGTTCAATGGTGTTGATTGGTGGGGATCCAGGCATTGGGAAATCAACCCTCTT
GCTCCAAGTTTCGGGACAATTGGCCCACGAAGGCCGTGTGTTATACGTGACTGGGGAAGAATCTGCCACCCAAGTAAAGT
TGCGGGCAGATCGCTTGGGTGTGGGGAATGATGAATTTTACCTCTATCCAGAAACAGATATGACGGCGATTAAAAAGCAA
ATTGAATCGCTGCAACCAGATTTTGTCGTGATTGACTCAGTGCAAACGATGCAGGAACCAGACGTGACGTCCGCCATTGG
CTCCGTGTCACAAATCCGTGAAGTCACGGCAGATTTATTGCAAATTGCCAAAACCAATAACATTTCTATTTTTATCGTTG
GTCACGTGACTAAAGATGGGGCAATTGCTGGACCAAAGATTTTGGAGCATATGGTCGATACGGTGCTCTATTTTGAAGGC
GATAGTAACTATAAGTACCGCATTTTACGCGCGGTGAAAAATCGTTTTGGTGCCACCAATGAATTGGGTATTTTTGAAAT
GCGTGACGGCGGTTTGATTGAAGTCGCCAACCCATCAGAAATCTTTTTGGAAGAACGATTAGCTGGCGCAACTGGTTCGG
CCATTGTGGTCGCCTTGGAAGGCTCACGGCCGATTTTAGTGGAGTTGCAAGCTTTGGTGACGCCAACCGTCTTTGGGAAT
GCGCAACGGACGGCATCCGGATTAGATCGTAACCGCGTGTCGCTAATTATGGCCGTTTTGGAAAAGCGCGCCAATCTCTT
GTTACAAAATCAAGATGCTTATCTGAAAGCTGCCGGTGGGGTCAAGCTGGATGAACCAGCAATTGATTTGGCGATTGCCG
TGGCGCTGGCCAGTTCTTATCACGATAAAGAATCGCGGCCAAGCGACGTCTTTGTCGGTGAAATCGGGTTAACTGGGGAA
ATCCGGAGCGTCGCTGATATTGAAAGTCGGTTGAAGGAAGCGCGCAAGCTCGGCTTTAAACGGGCGATTGTGCCAAAGAA
CAACTTGAATGGTGTTACCTTACCCGAAGGGATTCAAGTAATTGGCGTCGCCACATTGCGGGAAGCGTTGCAATTAGCCT
TAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8TEG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

66.962

99.121

0.664
  radA Streptococcus pneumoniae D39

66.962

99.121

0.664
  radA Streptococcus pneumoniae R6

66.962

99.121

0.664
  radA Streptococcus pneumoniae TIGR4

66.962

99.121

0.664
  radA Streptococcus mitis NCTC 12261

66.741

99.121

0.662
  radA Streptococcus mitis SK321

66.741

99.121

0.662
  radA Bacillus subtilis subsp. subtilis str. 168

62.281

100

0.624

Multiple sequence alignment