Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E0F31_RS00105 Genome accession   NZ_LR216064
Coordinates   10556..11917 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5556..16917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS00050 (SAMEA3714520_00009) - 6209..6589 (-) 381 WP_000170931.1 ImmA/IrrE family metallo-endopeptidase -
  E0F31_RS00055 (SAMEA3714520_00010) - 6602..6865 (-) 264 WP_000285962.1 type II toxin-antitoxin system RelE family toxin -
  E0F31_RS00060 (SAMEA3714520_00011) - 6865..7098 (-) 234 WP_000156419.1 hypothetical protein -
  E0F31_RS00065 (SAMEA3714520_00012) - 7098..7466 (-) 369 WP_000464160.1 helix-turn-helix domain-containing protein -
  E0F31_RS11985 (SAMEA3714520_00013) - 7763..7894 (+) 132 WP_000253628.1 hypothetical protein -
  E0F31_RS00075 (SAMEA3714520_00014) - 8184..8375 (+) 192 WP_001112859.1 DNA-binding protein -
  E0F31_RS00080 (SAMEA3714520_00015) - 8398..8601 (+) 204 WP_001247549.1 hypothetical protein -
  E0F31_RS00085 (SAMEA3714520_00017) - 8756..8923 (-) 168 WP_000024181.1 YjzC family protein -
  E0F31_RS00090 (SAMEA3714520_00018) - 8928..9308 (+) 381 Protein_14 autolysin -
  E0F31_RS00095 (SAMEA3714520_00019) - 9582..10025 (+) 444 WP_000701990.1 dUTP diphosphatase -
  E0F31_RS00100 (SAMEA3714520_00020) - 10027..10542 (+) 516 WP_000691237.1 histidine phosphatase family protein -
  E0F31_RS00105 (SAMEA3714520_00021) radA 10556..11917 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  E0F31_RS00110 (SAMEA3714520_00022) - 11990..12487 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  E0F31_RS00115 (SAMEA3714520_00023) - 12512..13327 (+) 816 WP_000749763.1 PrsW family intramembrane metalloprotease -
  E0F31_RS00120 (SAMEA3714520_00024) - 13472..14440 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  E0F31_RS00125 - 14574..14855 (-) 282 Protein_21 transposase family protein -
  E0F31_RS11580 - 14982..15889 (-) 908 Protein_22 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=1003929 E0F31_RS00105 WP_074017595.1 10556..11917(+) (radA) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1003929 E0F31_RS00105 WP_074017595.1 10556..11917(+) (radA) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACCACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA/sms Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment