Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   E0F32_RS00865 Genome accession   NZ_LR216050
Coordinates   144303..146252 (+) Length   649 a.a.
NCBI ID   WP_000018169.1    Uniprot ID   P0A3R1
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 139303..151252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS00830 (SAMEA3431333_00154) - 140256..140789 (+) 534 WP_000729030.1 hypothetical protein -
  E0F32_RS00835 (SAMEA3431333_00155) nrdI 140867..141337 (+) 471 WP_000849621.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  E0F32_RS00845 (SAMEA3431333_00156) - 141630..142892 (-) 1263 WP_000075789.1 NRAMP family divalent metal transporter -
  E0F32_RS00850 (SAMEA3431333_00157) - 143202..143660 (-) 459 WP_000745054.1 DUF3021 domain-containing protein -
  E0F32_RS00855 (SAMEA3431333_00158) - 143657..144097 (-) 441 WP_000861955.1 LytTR family DNA-binding domain-containing protein -
  E0F32_RS00865 (SAMEA3431333_00159) hexB 144303..146252 (+) 1950 WP_000018169.1 DNA mismatch repair endonuclease MutL Machinery gene
  E0F32_RS00870 (SAMEA3431333_00160) ribE 146577..147044 (-) 468 WP_001099505.1 6,7-dimethyl-8-ribityllumazine synthase -
  E0F32_RS00875 (SAMEA3431333_00161) - 147045..148250 (-) 1206 WP_000456639.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  E0F32_RS00880 (SAMEA3431333_00162) ribE 148270..148905 (-) 636 WP_000493842.1 riboflavin synthase -
  E0F32_RS00885 (SAMEA3431333_00163) ribD 148890..149990 (-) 1101 WP_001284106.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
  E0F32_RS00890 (SAMEA3431333_00164) ruvA 150396..150989 (+) 594 WP_000271490.1 Holliday junction branch migration protein RuvA Machinery gene

Sequence

Protein


Download         Length: 649 a.a.        Molecular weight: 73434.89 Da        Isoelectric Point: 5.3001

>NTDB_id=1003697 E0F32_RS00865 WP_000018169.1 144303..146252(+) (hexB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQIIIEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSK
IKNQADLFRIRTLGFRGEALPSIASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSAKKMIEIENSDLDFEISGFVSLP
ELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKLMVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSE
AIANSLKEQTLIPDALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLTLFAKETLD
RLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELEFFGQMHGTYLFAQGRDGLYIIDQHAAQERV
KYEEYRESIGNVDQSQQQLLVPYIFEFPADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCD
MLLLTKEVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHFTKSDMEKMFRRIQENH
TSLRELGKY

Nucleotide


Download         Length: 1950 bp        

>NTDB_id=1003697 E0F32_RS00865 WP_000018169.1 144303..146252(+) (hexB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGTCTCATATTATTGAATTGCCAGAGATGCTGGCAAACCAAATCGCAGCTGGAGAGGTCATTGAACGTCCTGCCAGTGT
GGTCAAAGAGTTGGTAGAAAATGCCATTGACGCGGGCTCTAGTCAGATTATCATTGAGATTGAGGAAGCTGGTCTCAAGA
AGGTTCAAATCACGGATAACGGTCATGGAATTGCCCACGATGAGGTGGAGTTGGCCCTGCGTCGCCATGCGACCAGTAAG
ATAAAAAATCAAGCAGATCTCTTTCGGATTCGGACGCTTGGTTTTCGTGGTGAAGCCTTGCCTTCTATTGCGTCTGTTAG
TGTCTTGACTCTGTTAACGGCGGTGGATGGTGCTAGTCATGGAACCAAGTTAGTCGCGCGTGGGGGTGAAGTTGAGGAAG
TCATCCCAGCGACTAGTCCTGTGGGAACCAAGGTTTGTGTGGAGGATCTCTTTTTCAACACGCCTGCCCGTCTCAAGTAT
ATGAAGAGCCAGCAAGCGGAGTTGTCTCATATCATTGATATTGTCAACCGTCTGGGCTTGGCCCATCCTGAGATTTCTTT
TAGCTTGATTAGTGATGGCAAGGAAATGACGCGGACAGCAGGGACTGGTCAACTGCGCCAAGCAATCGCAGGGATTTACG
GTTTGGTCAGTGCCAAGAAGATGATTGAAATTGAGAACTCTGACCTAGATTTCGAAATTTCAGGTTTTGTGTCTTTACCT
GAGTTGACTCGGGCTAACCGCAATTATATCAGCCTCTTCATCAATGGCCGTTATATCAAGAACTTCCTGCTAAATCGTGC
TATTTTGGATGGTTTCGGAAGCAAGCTTATGGTTGGACGTTTTCCACTGGCTGTCATTCACATCCATATCGACCCTTATC
TAGCGGATGTCAATGTGCATCCAACTAAGCAAGAGGTGCGGATTTCCAAGGAAAAAGAACTGATGACCCTGGTTTCAGAA
GCTATTGCAAATAGTCTCAAGGAACAAACCTTGATTCCAGATGCCTTGGAAAATCTTGCCAAATCGACCGTGCGCAATCG
TGAGAAGGTGGAGCAAACTATTCTCCCACTCAAAGAAAATACGCTCTACTATGAGAAAACTGAGCCGTCAAGACCTAGTC
AAACTGAAGTAGCTGATTATCAGGTAGAATTGACTGATGAAGGGCAGGATTTGACCCTGTTTGCCAAGGAAACCTTGGAC
CGATTGACCAAGCCAGCAAAACTGCATTTTGCAGAGAGAAAGCCTGCTAACTACGACCAGCTAGACCATCCAGAGTTAGA
TCTTGCTAGCATCGATAAGGCTTATGACAAACTGGAGCGAGAAGAAGCATCCAGCTTCCCAGAGTTGGAGTTTTTCGGAC
AAATGCACGGGACTTATCTCTTTGCCCAAGGGCGAGATGGACTTTACATCATAGATCAGCACGCTGCTCAGGAACGGGTC
AAGTACGAGGAGTACCGTGAAAGCATTGGCAATGTTGACCAGAGCCAGCAGCAACTCCTAGTGCCCTATATCTTTGAATT
TCCTGCGGATGATGCCCTGCGTCTCAAGGAAAGAATGCCTCTCTTAGAGGAAGTGGGCGTCTTTCTAGCAGAGTACGGAG
AAAATCAATTTATTCTACGTGAACATCCTATTTGGATGGCAGAAGAAGAGATTGAATCAGGCATCTATGAGATGTGCGAC
ATGCTCCTTTTGACCAAGGAAGTTTCTATCAAGAAATACCGAGCAGAGCTGGCTATCATGATGTCTTGCAAGCGATCTAT
CAAGGCCAATCATCGTATTGATGATCATTCAGCTAGACAACTCCTCTATCAGCTTTCTCAATGTGACAATCCCTATAACT
GTCCTCACGGACGTCCTGTTTTGGTGCATTTTACCAAGTCGGATATGGAAAAGATGTTCCGACGTATTCAGGAAAATCAC
ACCAGTCTCCGTGAGTTGGGGAAATATTAA

Domains

Predicted by InterProScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-107)

MutL C terminal dimerisation domain (MutL_C)

(450-592)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A3R1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

100

100

1