Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   ECRM13516_RS21380 Genome accession   NZ_CP006262
Coordinates   4190866..4191630 (+) Length   254 a.a.
NCBI ID   WP_001136214.1    Uniprot ID   -
Organism   Escherichia coli O145:H28 str. RM13516     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4185866..4196630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECRM13516_RS21360 (ECRM13516_4246) acpT 4186820..4187407 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  ECRM13516_RS21365 (ECRM13516_4247) nikA 4187518..4189092 (+) 1575 WP_000953365.1 nickel ABC transporter substrate-binding protein -
  ECRM13516_RS21370 (ECRM13516_4248) nikB 4189092..4190036 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  ECRM13516_RS21375 (ECRM13516_4249) nikC 4190033..4190866 (+) 834 WP_001008970.1 nickel ABC transporter permease subunit NikC -
  ECRM13516_RS21380 (ECRM13516_4250) amiE 4190866..4191630 (+) 765 WP_001136214.1 nickel import ATP-binding protein NikD Regulator
  ECRM13516_RS21385 (ECRM13516_4251) nikE 4191627..4192433 (+) 807 WP_000173674.1 nickel import ATP-binding protein NikE -
  ECRM13516_RS21390 (ECRM13516_4252) nikR 4192439..4192840 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  ECRM13516_RS21395 (ECRM13516_4253) - 4193039..4193785 (+) 747 WP_001603991.1 GntR family transcriptional regulator -
  ECRM13516_RS21400 (ECRM13516_4254) - 4193810..4194283 (+) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  ECRM13516_RS21405 (ECRM13516_4255) - 4194280..4194561 (+) 282 WP_000084022.1 PTS sugar transporter subunit IIB -
  ECRM13516_RS21410 (ECRM13516_4256) - 4194638..4195996 (+) 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26809.44 Da        Isoelectric Point: 6.7450

>NTDB_id=100301 ECRM13516_RS21380 WP_001136214.1 4190866..4191630(+) (amiE) [Escherichia coli O145:H28 str. RM13516]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLRTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=100301 ECRM13516_RS21380 WP_001136214.1 4190866..4191630(+) (amiE) [Escherichia coli O145:H28 str. RM13516]
ATGCCGCAACAGATTGAACTGCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTAGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACGCACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACTGACCTCGATGTAGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCACCGGGGATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment