Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEF79_RS02550 Genome accession   NZ_CP156810
Coordinates   536871..537671 (+) Length   266 a.a.
NCBI ID   WP_347453149.1    Uniprot ID   -
Organism   Acinetobacter sp. ANC 7454     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531871..542671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF79_RS02520 (ABEF79_02520) tnpB 532137..532472 (+) 336 WP_000618091.1 IS66 family insertion sequence element accessory protein TnpB -
  ABEF79_RS02525 (ABEF79_02525) - 532547..534172 (+) 1626 WP_347454390.1 IS66 family transposase -
  ABEF79_RS02530 (ABEF79_02530) - 534351..534668 (+) 318 Protein_487 phosphatase PAP2 family protein -
  ABEF79_RS02535 (ABEF79_02535) iscX 534756..534956 (+) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  ABEF79_RS02540 (ABEF79_02540) ndk 535067..535498 (+) 432 WP_034587246.1 nucleoside-diphosphate kinase -
  ABEF79_RS02545 (ABEF79_02545) rlmN 535621..536856 (+) 1236 WP_347453150.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEF79_RS02550 (ABEF79_02550) pilF 536871..537671 (+) 801 WP_347453149.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEF79_RS02555 (ABEF79_02555) - 537662..538450 (+) 789 WP_347453148.1 RodZ domain-containing protein -
  ABEF79_RS02560 (ABEF79_02560) ispG 538474..539595 (+) 1122 WP_347460908.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEF79_RS02565 (ABEF79_02565) hisS 539599..540894 (+) 1296 WP_347453146.1 histidine--tRNA ligase -
  ABEF79_RS02570 (ABEF79_02570) - 540925..541623 (+) 699 WP_347453145.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.39 Da        Isoelectric Point: 9.3896

>NTDB_id=1002995 ABEF79_RS02550 WP_347453149.1 536871..537671(+) (pilF) [Acinetobacter sp. ANC 7454]
MTKMISKIALISTVCFSLALNACQTVDAGKKDPEKAIKVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSKANLEKAESYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFEIAGATLGYDQRYRALENLGRIYLKKGDL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASKMYEQFVHAVGQKNQGARALWIGIRTARASGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1002995 ABEF79_RS02550 WP_347453149.1 536871..537671(+) (pilF) [Acinetobacter sp. ANC 7454]
TTGACAAAGATGATATCTAAAATTGCTTTAATTTCAACAGTATGTTTTTCACTTGCATTGAATGCCTGTCAGACGGTGGA
CGCGGGGAAAAAGGACCCAGAAAAGGCAATTAAGGTACGTACTCAATTGGCGGTTGAATACCTGAAAACAGGTGATCTGG
ATTCAGCCAAGCGCGCACTGGACGAAGCACTGAAAATCAATTCACGTGATGCCCAGGCCAATATGATGATGGGTGTGCTG
CTCCAACAGGAAGGCAGCAAGGCCAATCTGGAAAAAGCGGAAAGTTATTTTAAAAAAGCGATTTCTGCTGAACCGAAAAA
TGCACAGGCGCGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAGTTTGAAATTG
CTGGCGCAACCCTGGGTTATGACCAACGTTACCGTGCCCTGGAAAATCTGGGGCGGATCTACCTGAAAAAAGGTGACCTG
GTAAATGCTGAAAAATCATTCAAACAGGCACTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAACCAGCAGTTCCCTGCAGCGAGCAAAATGTATGAACAGTTTGTGCATGCGGTAGGGCAGAAGAATCAGG
GTGCCCGCGCACTCTGGATAGGCATTCGCACAGCGCGTGCCAGTGGCGATCAACTCGGAATGCAGGTACTGGTGAATCAG
CTGCGTGCACTTTTCCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_8)

(38-68)

Tetratricopeptide repeat (TPR_17)

(97-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

97.744

0.771
  pilF Acinetobacter baylyi ADP1

68.702

98.496

0.677

Multiple sequence alignment