Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AACH30_RS00925 Genome accession   NZ_AP027243
Coordinates   172830..174191 (+) Length   453 a.a.
NCBI ID   WP_265150865.1    Uniprot ID   A0AA43PGI0
Organism   Lactococcus garvieae strain L21-68     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 167830..179191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH30_RS00880 (LG21E68_01660) - 168294..169016 (-) 723 WP_003133770.1 metal ABC transporter ATP-binding protein -
  AACH30_RS00885 (LG21E68_01670) - 169038..169481 (-) 444 WP_014024192.1 zinc-dependent MarR family transcriptional regulator -
  AACH30_RS00890 (LG21E68_01680) infA 169683..169901 (+) 219 WP_003133772.1 translation initiation factor IF-1 -
  AACH30_RS00895 rpmJ 169955..170071 (+) 117 WP_014024193.1 50S ribosomal protein L36 -
  AACH30_RS00900 (LG21E68_01690) rpsM 170089..170454 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  AACH30_RS00905 (LG21E68_01700) rpsK 170472..170855 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  AACH30_RS00910 (LG21E68_01710) - 170907..171845 (+) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  AACH30_RS00915 (LG21E68_01720) rplQ 171861..172241 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  AACH30_RS00925 (LG21E68_01730) radA 172830..174191 (+) 1362 WP_265150865.1 DNA repair protein RadA Machinery gene
  AACH30_RS00930 (LG21E68_01740) - 174325..175401 (+) 1077 WP_023889408.1 PIN/TRAM domain-containing protein -
  AACH30_RS00935 (LG21E68_01750) - 175424..176053 (+) 630 WP_265150047.1 pyridoxamine 5'-phosphate oxidase family protein -
  AACH30_RS00940 (LG21E68_01760) ald 176242..177363 (+) 1122 WP_141921055.1 alanine dehydrogenase -
  AACH30_RS00945 (LG21E68_01770) - 177397..177891 (-) 495 WP_014024197.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49796.98 Da        Isoelectric Point: 4.8676

>NTDB_id=100292 AACH30_RS00925 WP_265150865.1 172830..174191(+) (radA) [Lactococcus garvieae strain L21-68]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLVGVDIPENLEVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=100292 AACH30_RS00925 WP_265150865.1 172830..174191(+) (radA) [Lactococcus garvieae strain L21-68]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAGGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTGAGTCTGACAGGCGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACTGATCTGGATGAGTTTAACCGCGTG
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGAGATCCTGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGGGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGGCCACGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACCAACCCGAGTGAAGTTTTTCTTGAGGAGCGTTTGGAGGGCTCTACAGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTAACAACGCCAACCATGTTTGGAAATGCCAAA
CGTACCACCTCTGGTTTGGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTCGAAAAACGTGCCGGTTTTCTTTTGCA
ACAACAAGATGCCTACTTAAAATCCGCAGGTGGTGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTCGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGCTTTATTGGTGAAATTGGCCTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAACTAGGTTTCCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGTAGGCGTAGATATTCCAGAAAATTTGGAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.353

100

0.804
  radA Streptococcus pneumoniae D39

80.353

100

0.804
  radA Streptococcus pneumoniae R6

80.353

100

0.804
  radA Streptococcus pneumoniae TIGR4

80.353

100

0.804
  radA Streptococcus mitis NCTC 12261

80.353

100

0.804
  radA Streptococcus mitis SK321

80.353

100

0.804
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623

Multiple sequence alignment