Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEF85_RS02520 Genome accession   NZ_CP156776
Coordinates   540456..541247 (+) Length   263 a.a.
NCBI ID   WP_404798875.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7955     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 535456..546247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF85_RS02495 (ABEF85_02495) - 537184..537594 (+) 411 WP_347454710.1 hypothetical protein -
  ABEF85_RS02500 (ABEF85_02500) - 537688..538242 (+) 555 WP_347464305.1 phosphatase PAP2 family protein -
  ABEF85_RS02505 (ABEF85_02505) iscX 538332..538532 (+) 201 WP_347456360.1 Fe-S cluster assembly protein IscX -
  ABEF85_RS02510 (ABEF85_02510) ndk 538643..539074 (+) 432 WP_347455986.1 nucleoside-diphosphate kinase -
  ABEF85_RS02515 (ABEF85_02515) rlmN 539197..540432 (+) 1236 WP_347455985.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEF85_RS02520 (ABEF85_02520) pilF 540456..541247 (+) 792 WP_404798875.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEF85_RS02525 (ABEF85_02525) - 541238..542023 (+) 786 WP_347454713.1 RodZ domain-containing protein -
  ABEF85_RS02530 (ABEF85_02530) ispG 542047..543168 (+) 1122 WP_347453147.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEF85_RS02535 (ABEF85_02535) hisS 543172..544467 (+) 1296 WP_347453146.1 histidine--tRNA ligase -
  ABEF85_RS02540 (ABEF85_02540) - 544498..545196 (+) 699 WP_347453145.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 263 a.a.        Molecular weight: 29712.89 Da        Isoelectric Point: 9.2335

>NTDB_id=1002625 ABEF85_RS02520 WP_404798875.1 540456..541247(+) (pilF) [Acinetobacter thermotolerans strain ANC 7955]
MTSKIALISTICFSLALTACQTVDAGKKDPEKAIKVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVLLQQ
EGSKANLEKAESYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFEIAGATLGYDQRYRALENLGRIYLKKGDLVNA
EKSFKQALQANRDSYISMLELAEIFYLNQQFPAASKMYEQFVHAVGQKNQGARALWIGIRTARASGDQLGMQVLVNQLRA
LFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 792 bp        

>NTDB_id=1002625 ABEF85_RS02520 WP_404798875.1 540456..541247(+) (pilF) [Acinetobacter thermotolerans strain ANC 7955]
ATGACATCTAAAATTGCTTTAATTTCAACAATATGTTTTTCACTTGCATTAACTGCCTGTCAGACAGTAGACGCGGGGAA
AAAGGACCCGGAAAAGGCAATTAAGGTACGTACTCAATTGGCGGTTGAATACCTGAAAACAGGTGATCTGGATTCAGCCA
AGCGCGCCCTGGACGAAGCACTGAAAATCAATTCACGTGATGCCCAGGCCAATATGATGATGGGCGTGCTGCTACAACAG
GAAGGCAGTAAAGCCAATCTGGAAAAAGCGGAAAGTTATTTTAAAAAAGCGATTTCTGCTGAGCCGAAAAATGCACAGGC
GCGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAGTTTGAAATTGCCGGTGCCA
CCCTGGGCTATGACCAGCGTTACCGTGCCCTGGAAAATCTGGGGCGGATTTACCTGAAAAAAGGGGATCTGGTAAATGCT
GAAAAATCATTCAAACAGGCACTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGATTTTTTATCT
GAACCAGCAGTTCCCTGCAGCGAGCAAAATGTATGAACAGTTTGTGCATGCGGTAGGGCAGAAGAATCAGGGTGCTCGTG
CACTCTGGATAGGTATTCGCACAGCGCGTGCCAGTGGCGATCAACTCGGAATGCAGGTACTGGTGAATCAGCTGCGTGCA
CTTTTCCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(35-65)

Tetratricopeptide repeat (TPR_8)

(142-173)

Tetratricopeptide repeat (TPR_17)

(94-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

98.859

0.776
  pilF Acinetobacter baylyi ADP1

70.196

96.958

0.681

Multiple sequence alignment