Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEF80_RS12480 Genome accession   NZ_CP156772
Coordinates   2554628..2555419 (-) Length   263 a.a.
NCBI ID   WP_404798956.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7970     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549628..2560419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF80_RS12460 (ABEF80_12460) - 2550676..2551374 (-) 699 WP_347453145.1 tetratricopeptide repeat protein -
  ABEF80_RS12465 (ABEF80_12465) hisS 2551405..2552700 (-) 1296 WP_347453146.1 histidine--tRNA ligase -
  ABEF80_RS12470 (ABEF80_12470) ispG 2552704..2553825 (-) 1122 WP_347453147.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEF80_RS12475 (ABEF80_12475) - 2553849..2554637 (-) 789 WP_347453148.1 RodZ domain-containing protein -
  ABEF80_RS12480 (ABEF80_12480) pilF 2554628..2555419 (-) 792 WP_404798956.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEF80_RS12485 (ABEF80_12485) rlmN 2555443..2556678 (-) 1236 WP_347453150.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEF80_RS12490 (ABEF80_12490) ndk 2556801..2557232 (-) 432 WP_034587246.1 nucleoside-diphosphate kinase -
  ABEF80_RS12495 (ABEF80_12495) iscX 2557343..2557543 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  ABEF80_RS12500 (ABEF80_12500) - 2557631..2558185 (-) 555 WP_347454179.1 phosphatase PAP2 family protein -
  ABEF80_RS12505 (ABEF80_12505) - 2558279..2558689 (-) 411 WP_347453151.1 hypothetical protein -

Sequence

Protein


Download         Length: 263 a.a.        Molecular weight: 29723.92 Da        Isoelectric Point: 9.2335

>NTDB_id=1002612 ABEF80_RS12480 WP_404798956.1 2554628..2555419(-) (pilF) [Acinetobacter thermotolerans strain ANC 7970]
MISKIALISTVCFSLALNACQTVDAGKKDPEKAIKVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVLLQQ
EGSKANLEKAESYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFEIAGATLGYDQRYRALENLGRIYLKKGDLVNA
EKSFKQALQANRDSYISMLELAEIFYLNQQFPAASKMYEQFVHAVGQKNQGARALWIGIRTARASGDQLGMQVLVNQLRA
LFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 792 bp        

>NTDB_id=1002612 ABEF80_RS12480 WP_404798956.1 2554628..2555419(-) (pilF) [Acinetobacter thermotolerans strain ANC 7970]
ATGATATCTAAAATTGCTTTAATTTCAACAGTATGTTTTTCACTTGCATTGAATGCCTGTCAGACGGTGGACGCGGGGAA
AAAGGACCCAGAAAAGGCAATTAAGGTACGTACTCAATTGGCGGTTGAATACCTGAAAACAGGTGATCTGGATTCAGCCA
AGCGCGCACTGGACGAAGCACTGAAAATCAATTCACGTGATGCCCAGGCCAATATGATGATGGGTGTGCTGCTCCAACAG
GAAGGCAGCAAGGCCAATCTGGAAAAAGCGGAAAGTTATTTTAAAAAAGCGATTTCTGCTGAACCGAAAAATGCACAGGC
GCGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAGTTTGAAATTGCTGGCGCAA
CCCTGGGTTATGACCAACGTTACCGTGCCCTGGAAAATCTGGGGCGGATCTACCTGAAAAAAGGTGACCTGGTAAATGCT
GAAAAATCATTCAAACAGGCACTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGATTTTTTATCT
GAACCAGCAGTTCCCTGCAGCGAGCAAAATGTATGAACAGTTTGTGCATGCGGTAGGGCAGAAGAATCAGGGTGCCCGCG
CACTCTGGATAGGCATTCGCACAGCGCGTGCCAGTGGCGATCAACTCGGAATGCAGGTACTGGTGAATCAGCTGCGTGCA
CTTTTCCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(94-126)

Tetratricopeptide repeat (TPR_8)

(35-65)

Tetratricopeptide repeat (TPR_8)

(142-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

98.859

0.779
  pilF Acinetobacter baylyi ADP1

68.702

99.62

0.684

Multiple sequence alignment