Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEF83_RS02440 Genome accession   NZ_CP156768
Coordinates   519967..520758 (+) Length   263 a.a.
NCBI ID   WP_161788388.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7974     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514967..525758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF83_RS02415 (ABEF83_02415) - 516697..517107 (+) 411 WP_347454710.1 hypothetical protein -
  ABEF83_RS02420 (ABEF83_02420) - 517201..517755 (+) 555 WP_347454179.1 phosphatase PAP2 family protein -
  ABEF83_RS02425 (ABEF83_02425) iscX 517843..518043 (+) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  ABEF83_RS02430 (ABEF83_02430) ndk 518154..518585 (+) 432 WP_034587246.1 nucleoside-diphosphate kinase -
  ABEF83_RS02435 (ABEF83_02435) rlmN 518708..519943 (+) 1236 WP_347454711.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEF83_RS02440 (ABEF83_02440) pilF 519967..520758 (+) 792 WP_161788388.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEF83_RS02445 (ABEF83_02445) - 520749..521534 (+) 786 WP_347454713.1 RodZ domain-containing protein -
  ABEF83_RS02450 (ABEF83_02450) ispG 521558..522679 (+) 1122 WP_347454714.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEF83_RS02455 (ABEF83_02455) hisS 522683..523978 (+) 1296 WP_347454715.1 histidine--tRNA ligase -
  ABEF83_RS02460 (ABEF83_02460) - 524009..524707 (+) 699 WP_347453145.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 263 a.a.        Molecular weight: 29698.87 Da        Isoelectric Point: 9.2335

>NTDB_id=1002542 ABEF83_RS02440 WP_161788388.1 519967..520758(+) (pilF) [Acinetobacter thermotolerans strain ANC 7974]
MTSKIALISTVCFSLALTACQTVDAGKKDPEKAIKVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVLLQQ
EGSKANLEKAESYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFEIAGATLGYDQRYRALENLGRIYLKKGDLVNA
EKSFKQALQANRDSYISMLELAEIFYLNQQFPAASKMYEQFVHAVGQKNQGARALWIGIRTARASGDQLGMQVLVNQLRA
LFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 792 bp        

>NTDB_id=1002542 ABEF83_RS02440 WP_161788388.1 519967..520758(+) (pilF) [Acinetobacter thermotolerans strain ANC 7974]
ATGACATCTAAAATTGCTTTAATTTCAACAGTATGTTTTTCACTTGCATTGACTGCCTGTCAGACGGTAGACGCGGGGAA
AAAGGACCCGGAAAAGGCAATTAAGGTACGTACTCAATTGGCGGTTGAATACCTGAAAACAGGTGATCTGGATTCAGCCA
AGCGTGCACTGGACGAAGCACTGAAAATCAATTCACGTGATGCCCAGGCCAATATGATGATGGGTGTGCTGCTCCAGCAG
GAAGGCAGTAAAGCCAATCTGGAAAAAGCGGAAAGTTATTTTAAAAAAGCGATTTCTGCTGAACCGAAAAATGCACAGGC
GCGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAGTTTGAAATTGCCGGTGCAA
CCCTGGGTTATGACCAGCGTTACCGTGCCCTGGAAAATCTGGGGCGGATCTACCTGAAAAAAGGGGATCTGGTAAATGCT
GAAAAATCATTCAAACAGGCACTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGATTTTTTATCT
GAACCAGCAGTTCCCTGCAGCGAGCAAAATGTATGAACAGTTTGTGCATGCGGTAGGGCAGAAGAATCAGGGTGCTCGTG
CACTCTGGATAGGCATTCGCACAGCGCGTGCCAGTGGCGATCAACTCGGAATGCAGGTACTGGTGAATCAGCTGCGTGCA
CTTTTCCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(94-126)

Tetratricopeptide repeat (TPR_8)

(35-65)

Tetratricopeptide repeat (TPR_8)

(142-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

98.859

0.779
  pilF Acinetobacter baylyi ADP1

69.804

96.958

0.677

Multiple sequence alignment