Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AACH63_RS01055 Genome accession   NZ_AP027240
Coordinates   192218..193579 (+) Length   453 a.a.
NCBI ID   WP_096368775.1    Uniprot ID   -
Organism   Lactococcus formosensis strain L21-20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 187218..198579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH63_RS01020 (LG21E20_01950) rpsM 187232..187597 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  AACH63_RS01025 (LG21E20_01960) rpsK 187615..187998 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  AACH63_RS01030 (LG21E20_01970) - 188050..188988 (+) 939 WP_096368778.1 DNA-directed RNA polymerase subunit alpha -
  AACH63_RS01035 (LG21E20_01980) rplQ 189004..189384 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  AACH63_RS01040 (LG21E20_01990) - 189776..191260 (+) 1485 WP_096368777.1 S-layer homology domain-containing protein -
  AACH63_RS01045 (LG21E20_02000) - 191270..191707 (+) 438 WP_096368776.1 hypothetical protein -
  AACH63_RS01055 (LG21E20_02010) radA 192218..193579 (+) 1362 WP_096368775.1 DNA repair protein RadA Machinery gene
  AACH63_RS01060 (LG21E20_02020) - 193714..194790 (+) 1077 WP_096368774.1 PIN/TRAM domain-containing protein -
  AACH63_RS01065 (LG21E20_02030) - 194799..195428 (+) 630 WP_096368773.1 hypothetical protein -
  AACH63_RS01070 (LG21E20_02040) - 195564..196070 (+) 507 WP_096368772.1 PepSY domain-containing protein -
  AACH63_RS01075 (LG21E20_02050) ald 196271..197392 (+) 1122 WP_017368774.1 alanine dehydrogenase -
  AACH63_RS01080 (LG21E20_02060) - 197423..197917 (-) 495 WP_017368775.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49672.84 Da        Isoelectric Point: 5.0724

>NTDB_id=100247 AACH63_RS01055 WP_096368775.1 192218..193579(+) (radA) [Lactococcus formosensis strain L21-20]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIGTFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGSGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARDCFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVSTPENLKVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=100247 AACH63_RS01055 WP_096368775.1 192218..193579(+) (radA) [Lactococcus formosensis strain L21-20]
ATAGCTAAAAAAAAATCAACATTTCTTTGTCAAGAATGTGGTTATAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGCGCTTGGGGCTCCTTTGTGGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTAAGTTTGACAGGTGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTTGATACACCGCGCGTGGAAACCGACCTAGATGAGTTTAACCGAGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGCGATCCAGGGATTGGTAAATCGACTCTACTGCTTCA
AGTATCTACACAACTTGCTTCAAGAGGGCGTGTCCTTTATGTGAGTGGGGAGGAGTCAGCTCAACAAATTAAGCTGCGTG
CTGAGCGTTTGGGCGACATTGATACAGATTTTTATCTTTATGCCGAAACCAATATGCAAAGTATCCGAAATGAAGTGGAA
CGTCTACAGCCAGATTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATTCAGAGTACCCAGGGTTCAGT
TAGTCAAGTGCGTGAGGTGACAGGAGAATTAATGCAATTGGCCAAAACTAACGATATTGGGACTTTTATTGTGGGACATG
TCACCAAGGAAGGGCAACTTGCAGGCCCACGTATGTTGGAACATATGGTTGATACCGTCTTGTATTTTGAAGGAGAACGC
CAAAATACATTCCGAATTTTACGTGCGGTCAAAAACCGCTTTGGTTCAACAAATGAAATTGGAATTTTCGAGATGCAAGG
CAGTGGTTTAGTGGAAGTAACCAACCCGAGTGAAGTCTTTCTGGAAGAACGTCTCGAGGGTTCTACCGGATCTGCTATTG
TTTGTGCATTAGAAGGTACTCGTCCAATATTAGTTGAAATTCAAGCTTTGACTACACCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCCGGTTTAGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTTGAAAAGCGGGCCGGATTTCTTTTGCA
GCAACAAGACGCTTACTTGAAGTCAGCAGGTGGCGTTAAATTGGATGAGCCTGCAATTGACTTAGCAGTTGCAGTAGCTA
TTGCTTCTAGCTACAAAGAAGAGCCAACAGATGCACGCGACTGCTTTATTGGTGAAATTGGCTTAACTGGTGAAATCCGT
CGTGTCACACGCATGGAGCAACGTCTTAATGAAGCGAGTAAATTAGGTTTCCAAAAAGTTTATGCGCCTAAGAATAGCTT
AGCAGGCGTAAGTACTCCAGAAAACTTGAAAGTGGTTGGTGTGACAACTTTATCTGAATGTTTGAAAAAAGTATTTGGTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

79.912

100

0.799
  radA Streptococcus pneumoniae D39

79.912

100

0.799
  radA Streptococcus pneumoniae R6

79.912

100

0.799
  radA Streptococcus pneumoniae TIGR4

79.912

100

0.799
  radA Streptococcus mitis NCTC 12261

79.912

100

0.799
  radA Streptococcus mitis SK321

79.912

100

0.799
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623

Multiple sequence alignment