Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABFO07_RS04515 Genome accession   NZ_CP156685
Coordinates   948986..950488 (-) Length   500 a.a.
NCBI ID   WP_059885326.1    Uniprot ID   A0A119RVH8
Organism   Burkholderia stagnalis strain YJ-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943986..955488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFO07_RS04490 - 944973..945611 (-) 639 WP_059803140.1 LON peptidase substrate-binding domain-containing protein -
  ABFO07_RS04495 rapZ 945638..946549 (-) 912 WP_059566551.1 RNase adapter RapZ -
  ABFO07_RS04500 hprK 946625..947593 (-) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  ABFO07_RS04505 - 947730..948242 (-) 513 WP_059566552.1 PTS sugar transporter subunit IIA -
  ABFO07_RS04510 raiA 948461..948829 (-) 369 WP_017334258.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ABFO07_RS04515 rpoN/rpoN1 948986..950488 (-) 1503 WP_059885326.1 RNA polymerase factor sigma-54 Machinery gene
  ABFO07_RS04520 lptB 950632..951408 (-) 777 WP_059566554.1 LPS export ABC transporter ATP-binding protein -
  ABFO07_RS04525 lptA 951405..952070 (-) 666 WP_059566555.1 lipopolysaccharide transport periplasmic protein LptA -
  ABFO07_RS04530 lptC 952091..952705 (-) 615 WP_059566556.1 LPS export ABC transporter periplasmic protein LptC -
  ABFO07_RS04535 - 952709..953245 (-) 537 WP_059566557.1 HAD family hydrolase -
  ABFO07_RS04540 kdsD 953245..954228 (-) 984 WP_059566558.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 54721.88 Da        Isoelectric Point: 4.8219

>NTDB_id=1002208 ABFO07_RS04515 WP_059885326.1 948986..950488(-) (rpoN/rpoN1) [Burkholderia stagnalis strain YJ-2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAS
NGTSQAGERAERDEPAAADEYDGYAAGDSGDGPQWNLDEFGRASGASDDDDLPPLQVQEASTSLRDHLSAQLRVTQAGPR
DRALVMFLIESLDDDGYLTATLDEVLADLPAELEVDCDELNAALALLHSFDPAGVGARTASECLKLQLLRLDPSPTRTLS
LEIVSQYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVPR
LRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGL
HESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVVARR
TVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=1002208 ABFO07_RS04515 WP_059885326.1 948986..950488(-) (rpoN/rpoN1) [Burkholderia stagnalis strain YJ-2]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTGGCGCTGACACCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCCACGCTCGAGCTCCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCAGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCCCAGAACACCGAGCCGGCATCG
AACGGCACCAGCCAGGCCGGCGAGCGCGCGGAGCGCGACGAGCCCGCCGCCGCCGATGAATACGACGGCTATGCCGCGGG
CGACAGCGGCGACGGCCCGCAATGGAATCTCGATGAATTCGGCCGCGCGAGCGGCGCGTCGGACGACGACGATCTCCCGC
CCCTGCAGGTGCAGGAAGCGTCGACGTCGTTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCGGGGCCGCGC
GACCGTGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGACTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCCGCCG
GGGTCGGCGCGCGCACGGCATCCGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTGTCG
CTCGAAATCGTGTCGCAATACCTCGAGCTGCTCGCCGCACGCGACTTCACGCGCCTGCGCAAGCACCTGAAGGCATCCGA
CGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAGCGG
ACTACGTCGTGCCCGACATCATCGTGAAGAAGGTGGGCCAGGGCTGGCAGGCGGAACTGAATCCGGAGGTCGTGCCGCGC
CTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTCAAGCAGCAGCT
TCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGATACGATCCTGCGTGTCGCGCAGGCTATTGTCGAAC
GTCAGAAGAATTTCTTTGCGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGCCTA
CACGAGTCGACTGTCAGCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTTCTT
CGGATCGCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACGGCCATCCGTGCCCTCATCAAGCAACTGATAGGAG
CAGAAGACCCAAAAACGCCACTTTCGGACAGCCGCATCGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGCCGC
ACGGTTGCGAAGTACCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-327)

Sigma-54, DNA binding domain (Sigma54_DBD)

(343-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A119RVH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.78

100

0.69

Multiple sequence alignment