Detailed information NTG1002104

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABFY42_RS13555
• Genome accession: NZ_CP156682
• Coordinates: 2733125..2733739 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain B115
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2728125..2738739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFY42_RS13525 (ABFY42_13525)	rpsT	2728428..2728694 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ABFY42_RS13530 (ABFY42_13530)	holA	2728711..2729752 (-)	1042	Protein_2621	DNA polymerase III subunit delta	-
ABFY42_RS13535 (ABFY42_13535)	-	2729792..2729943 (-)	152	Protein_2622	hypothetical protein	-
ABFY42_RS13540 (ABFY42_13540)	-	2729984..2730118 (+)	135	WP_003152870.1	YqzM family protein	-
ABFY42_RS13545 (ABFY42_13545)	comEC	2730137..2732488 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABFY42_RS13550 (ABFY42_13550)	-	2732489..2733058 (-)	570	WP_003152868.1	ComE operon protein 2	-
ABFY42_RS13555 (ABFY42_13555)	comEA	2733125..2733739 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ABFY42_RS13560 (ABFY42_13560)	comER	2733798..2734619 (+)	822	WP_014418420.1	late competence protein ComER	-
ABFY42_RS13565 (ABFY42_13565)	-	2734688..2735425 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
ABFY42_RS13570 (ABFY42_13570)	rsfS	2735422..2735778 (-)	357	WP_007408260.1	ribosome silencing factor	-
ABFY42_RS13575 (ABFY42_13575)	yqeK	2735796..2736356 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABFY42_RS13580 (ABFY42_13580)	-	2736346..2736915 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ABFY42_RS13585 (ABFY42_13585)	yhbY	2736926..2737216 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ABFY42_RS13590 (ABFY42_13590)	aroE	2737210..2738052 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1002104 ABFY42_RS13555 WP_014418419.1 2733125..2733739(-) (comEA) [Bacillus velezensis strain B115]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1002104 ABFY42_RS13555 WP_014418419.1 2733125..2733739(-) (comEA) [Bacillus velezensis strain B115]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363