Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AACH29_RS01015 Genome accession   NZ_AP027239
Coordinates   185735..187096 (+) Length   453 a.a.
NCBI ID   WP_080567145.1    Uniprot ID   -
Organism   Lactococcus garvieae strain L21-12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 180735..192096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH29_RS00980 (LG21E12_01870) - 180859..181368 (-) 510 WP_338659878.1 helix-turn-helix transcriptional regulator -
  AACH29_RS00985 (LG21E12_01880) - 181599..181865 (+) 267 WP_338659879.1 hypothetical protein -
  AACH29_RS00990 (LG21E12_01890) - 182543..183769 (+) 1227 WP_338659880.1 replication initiation factor domain-containing protein -
  AACH29_RS00995 (LG21E12_01900) - 183766..183999 (+) 234 WP_338659881.1 hypothetical protein -
  AACH29_RS01000 (LG21E12_01910) - 184025..184243 (+) 219 WP_285016224.1 DUF3173 family protein -
  AACH29_RS01005 (LG21E12_01920) - 184221..185339 (+) 1119 WP_338659882.1 site-specific integrase -
  AACH29_RS01015 (LG21E12_01930) radA 185735..187096 (+) 1362 WP_080567145.1 DNA repair protein RadA Machinery gene
  AACH29_RS01020 (LG21E12_01940) - 187230..188306 (+) 1077 WP_023889408.1 PIN/TRAM domain-containing protein -
  AACH29_RS01025 (LG21E12_01950) - 188329..188958 (+) 630 WP_023889409.1 pyridoxamine 5'-phosphate oxidase family protein -
  AACH29_RS01030 (LG21E12_01960) ald 189146..190267 (+) 1122 WP_014024196.1 alanine dehydrogenase -
  AACH29_RS01035 (LG21E12_01970) - 190302..190796 (-) 495 WP_014024197.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49768.92 Da        Isoelectric Point: 4.8676

>NTDB_id=100204 AACH29_RS01015 WP_080567145.1 185735..187096(+) (radA) [Lactococcus garvieae strain L21-12]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLEVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=100204 AACH29_RS01015 WP_080567145.1 185735..187096(+) (radA) [Lactococcus garvieae strain L21-12]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAGGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTGAGTCTGACAGGCGAGCACT
CTAAGCCCATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACTGATCTGGATGAGTTTAACCGCGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGGGATCCAGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGGGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGGCCACGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACCAACCCGAGTGAAGTTTTTCTCGAAGAACGTTTGGAGGGCTCTACGGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTAACAACGCCAACCATGTTTGGAAATGCCAAA
CGTACAACCTCTGGTTTGGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTCGAAAAGCGTGCCGGTTTTCTTTTGCA
ACAACAAGATGCCTACTTAAAATCCGCAGGTGGCGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTCGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGCTTTATTGGCGAAATTGGCTTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAACTAGGTTTTCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGCAGGCGTAGACATTCCAGAAAATTTGGAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.353

100

0.804
  radA Streptococcus pneumoniae D39

80.353

100

0.804
  radA Streptococcus pneumoniae R6

80.353

100

0.804
  radA Streptococcus pneumoniae TIGR4

80.353

100

0.804
  radA Streptococcus mitis NCTC 12261

80.353

100

0.804
  radA Streptococcus mitis SK321

80.353

100

0.804
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623

Multiple sequence alignment