Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL140_RS00135 Genome accession   NZ_LR134336
Coordinates   23588..24949 (+) Length   453 a.a.
NCBI ID   WP_078232941.1    Uniprot ID   -
Organism   Streptococcus oralis ATCC 35037 strain NCTC 11427     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 18588..29949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL140_RS09680 (NCTC11427_00020) comW 19969..20205 (+) 237 WP_000939512.1 sigma(X)-activator ComW Regulator
  EL140_RS00110 (NCTC11427_00021) - 20447..21733 (+) 1287 WP_000205048.1 adenylosuccinate synthase -
  EL140_RS00115 (NCTC11427_00022) tadA 21934..22401 (+) 468 WP_001110101.1 tRNA adenosine(34) deaminase TadA -
  EL140_RS00125 (NCTC11427_00024) - 22590..23033 (+) 444 WP_000701998.1 dUTP diphosphatase -
  EL140_RS00130 (NCTC11427_00025) - 23035..23574 (+) 540 WP_002874984.1 histidine phosphatase family protein -
  EL140_RS00135 (NCTC11427_00026) radA 23588..24949 (+) 1362 WP_078232941.1 DNA repair protein RadA Machinery gene
  EL140_RS00140 (NCTC11427_00027) - 25022..25516 (+) 495 WP_002875379.1 beta-class carbonic anhydrase -
  EL140_RS00145 (NCTC11427_00028) - 25730..26698 (+) 969 WP_000010177.1 ribose-phosphate diphosphokinase -
  EL140_RS00150 (NCTC11427_00029) - 26818..27255 (+) 438 WP_000076475.1 CoA-binding protein -
  EL140_RS00155 (NCTC11427_00030) - 27284..28294 (-) 1011 WP_000009125.1 YeiH family protein -
  EL140_RS00160 (NCTC11427_00031) - 28446..29615 (+) 1170 WP_000366327.1 pyridoxal phosphate-dependent aminotransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49473.96 Da        Isoelectric Point: 5.8766

>NTDB_id=1001948 EL140_RS00135 WP_078232941.1 23588..24949(+) (radA) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
MAKKKATFVCQNCEYNSPKYLGRCPNCGSWSSFVEEVEIAEVKNARVSLAGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPELSGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIEVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1001948 EL140_RS00135 WP_078232941.1 23588..24949(+) (radA) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGAGTACAACTCACCTAAGTATTTAGGACGCTGTCCAAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTAGAGATTGCAGAGGTCAAGAATGCGCGTGTGTCCTTGGCAGGTGAGAAAA
CCAAGCCTATGAAACTGGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTA
CTTGGAGGCGGAGTGGTTCCAGGAAGTCTCGTCCTAATTGGGGGAGATCCTGGAATCGGAAAATCAACTCTTCTCCTACA
AGTTTCAACCCAGTTATCTCAAGTTGGTACGGTTCTCTATGTCAGTGGGGAGGAATCTGCCCAGCAGATTAAACTCCGTG
CAGAGCGCTTGGGGGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAGGTGGAG
CGCATCCAACCAGACTTTCTCATTATTGACTCTATCCAGACGATTATGTCTCCTGAGCTTTCAGGGGTACAAGGGTCTGT
TTCTCAGGTGCGTGAGGTGACAGCTGAGCTCATGCAGCTGGCCAAGACTAATAACATTGCCATCTTTATCGTAGGGCATG
TGACCAAGGAAGGTACTCTGGCCGGCCCGCGTATGTTGGAGCATATGGTAGATACGGTGCTTTACTTTGAAGGGGAACGC
CACCATACCTTCCGTATCTTGAGAGCAGTCAAAAACCGTTTTGGTTCCACTAATGAGATCGGCATCTTTGAGATGCAGTC
AGGTGGATTGGTTGAGGTGCTCAATCCGAGTCAAGTTTTTCTAGAAGAGCGTTTGGATGGAGCAACTGGTTCGTCAATCG
TGGTAACTATGGAAGGGACACGTCCGATTTTGGCGGAAGTGCAAGCCTTGGTGACACCGACTATGTTTGGGAATGCTAAG
CGCACGACGACAGGACTTGATTTCAATCGTGCAAGTCTGATTATGGCTGTTTTGGAAAAACGAGCAGGGCTTCTTTTGCA
AAACCAGGATGCCTATCTCAAATCTGCTGGTGGTGTAAAATTGGATGAGCCTGCCATTGACTTAGCCGTTGCAGTGGCTA
TTGCCTCTAGTTACAAGGACAAGCCTACCAATCCTCAGGAATGTTTTGTGGGTGAACTGGGCTTGACCGGAGAAATTCGA
CGCGTGAATCGTATCGAACAACGTATCAATGAAGCGGCAAAATTGGGCTTTACCAAGATTTATGTACCCAAGAATTCCTT
GACAGGAATCACTCCACCCAAGGAAATTGAAGTCATTGGTGTGACAACGATTCAGGAAGTTTTGAAGAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(63-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

98.675

100

0.987
  radA Streptococcus mitis NCTC 12261

98.675

100

0.987
  radA Streptococcus pneumoniae Rx1

98.675

100

0.987
  radA Streptococcus pneumoniae D39

98.675

100

0.987
  radA Streptococcus pneumoniae R6

98.675

100

0.987
  radA Streptococcus pneumoniae TIGR4

98.675

100

0.987
  radA/sms Bacillus subtilis subsp. subtilis str. 168

62.914

100

0.629