Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   EL144_RS05440 Genome accession   NZ_LR134327
Coordinates   1127173..1129371 (-) Length   732 a.a.
NCBI ID   WP_050332899.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1122173..1134371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL144_RS05425 (NCTC5906_01097) - 1124210..1125406 (-) 1197 WP_005704512.1 efflux RND transporter periplasmic adaptor subunit -
  EL144_RS05430 (NCTC5906_01098) - 1125444..1126010 (-) 567 WP_032995308.1 TetR/AcrR family transcriptional regulator -
  EL144_RS05435 (NCTC5906_01099) ftsN 1126215..1127069 (-) 855 WP_050332900.1 cell division protein FtsN -
  EL144_RS05440 (NCTC5906_01100) priA 1127173..1129371 (-) 2199 WP_050332899.1 primosomal protein N' Machinery gene
  EL144_RS05445 (NCTC5906_01101) - 1129681..1130391 (+) 711 WP_050332897.1 hypothetical protein -
  EL144_RS05450 (NCTC5906_01102) - 1130408..1131325 (+) 918 WP_005701787.1 choline kinase family protein -
  EL144_RS11550 (NCTC5906_01103) - 1131794..1132210 (+) 417 WP_232010651.1 EamA family transporter -
  EL144_RS05460 (NCTC5906_01104) - 1132207..1132905 (+) 699 WP_005704504.1 CTP--phosphocholine cytidylyltransferase -
  EL144_RS05465 (NCTC5906_01105) - 1133031..1133960 (-) 930 WP_005704503.1 capsular polysaccharide synthesis protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82203.40 Da        Isoelectric Point: 8.3866

>NTDB_id=1001740 EL144_RS05440 WP_050332899.1 1127173..1129371(-) (priA) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
MPFARIALPVPLHRYFDYVFPASMKVVVGGRVLVPFGTQKRVGVVVDVVEQTEVPEEQLKPVLASLDETSLFSPEIWLFL
NWAANYYHAPLGEVLSQALPVKLRQGESAVEKQIIFWKPTALGEQALVQGLLKRSKKQLEALQAANDGMEKGNNPFGSGI
WSALKAKEYITEIAQELEIQTWQQQLAGEPIVNKADRLTLNKQQALVFSQLKFTQGFAAWLLDGVTGSGKTEIYLQFIEE
ILNQGKQVLVLVPEIGLTPQTVSRFQARFNVVIDVLHSNLNDTQRLHVWQRARSGQSAVIIGTRSALFTQFADLGAIIID
EEHDASFKQQDGWRYHARDLAVVYAKQLNIPVLMGSATPSLESLNNVQQGKYQRLCLSEKANHSTALQQQIIDLKHQSMR
NGLSEILLKRMQAHLEKGNQVLLFLNRRGFAPVLLCHECGWMAECRHCDKPYTYHQQHNVLRCHHCSAQKPIPRQCGNCG
STQLITTGLGTEQLEDTLKAVFPNYGIARIDRDTTARKGKLEGYLADIQQGKSQILIGTQMLAKGHHFPNVTLVALVNVD
SALFSLDFRAEERLAQLYMQVAGRAGRGAQQGEVLLQTHYPEHPLLQSLLKHGYDNFAQQALALRHKMGLPPFSSQALFK
AQSRHSEDAEQLLAQFAEYFYGVKQHTAPELQILGPITAPFSKKAGQYRWQLLLQHPSRPILQQVLNEFQTSELNKPAQV
RWILDVDPLDLG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=1001740 EL144_RS05440 WP_050332899.1 1127173..1129371(-) (priA) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
ATGCCATTTGCCCGAATCGCACTTCCGGTGCCGCTACATCGCTATTTTGACTATGTCTTCCCTGCGTCAATGAAGGTTGT
TGTTGGGGGGCGGGTGCTTGTGCCGTTTGGGACGCAGAAGCGGGTTGGCGTTGTAGTTGACGTAGTGGAACAGACGGAAG
TGCCTGAAGAACAGTTGAAGCCTGTGTTAGCCTCTCTGGATGAAACTTCGCTGTTTTCACCGGAAATTTGGCTGTTCTTA
AATTGGGCAGCAAATTATTATCATGCGCCTTTGGGAGAAGTGTTATCCCAAGCATTGCCGGTGAAGCTACGTCAAGGTGA
AAGTGCGGTGGAAAAACAAATAATTTTTTGGAAACCGACCGCACTTGGTGAACAAGCGTTGGTGCAAGGTTTGCTAAAAC
GCTCGAAAAAACAGCTTGAAGCATTGCAGGCGGCAAATGATGGGATGGAAAAAGGCAACAACCCATTTGGTAGCGGCATT
TGGTCGGCGTTGAAAGCTAAAGAATACATTACCGAAATCGCTCAAGAACTGGAAATTCAAACCTGGCAACAACAATTAGC
CGGTGAACCAATCGTGAATAAAGCCGATCGATTAACACTCAACAAACAACAAGCGTTGGTGTTTAGCCAATTGAAATTTA
CCCAAGGGTTTGCAGCATGGTTGTTGGACGGCGTGACAGGTTCTGGCAAAACAGAAATCTATCTGCAATTTATTGAAGAG
ATTTTAAATCAAGGCAAACAAGTGTTGGTCTTGGTGCCGGAAATCGGGCTAACGCCACAAACGGTAAGTCGTTTCCAAGC
CCGTTTTAACGTGGTGATTGATGTGTTGCATTCCAATTTAAATGACACGCAACGATTACACGTGTGGCAACGTGCGCGTA
GCGGGCAAAGTGCGGTCATTATTGGTACGCGATCAGCGCTGTTTACGCAATTTGCCGATTTAGGCGCGATTATTATCGAT
GAAGAACATGACGCTTCTTTTAAGCAGCAAGACGGTTGGCGTTATCATGCTCGCGATTTGGCGGTGGTGTATGCAAAACA
ACTGAATATCCCCGTTTTAATGGGGTCGGCAACACCCAGTTTGGAAAGTCTGAATAATGTGCAACAGGGAAAATATCAGC
GTTTATGTCTGTCAGAAAAAGCCAATCATTCCACCGCACTTCAACAGCAAATTATTGATTTAAAACATCAGTCAATGCGC
AACGGTTTATCGGAAATCCTGTTAAAACGCATGCAGGCGCATTTAGAAAAAGGCAATCAAGTCTTGTTATTTCTAAATCG
ACGCGGTTTTGCACCGGTGTTGCTGTGTCATGAATGCGGTTGGATGGCGGAATGTCGCCATTGTGATAAGCCTTACACAT
ATCATCAACAACATAATGTCTTGCGTTGTCATCATTGCAGTGCGCAAAAGCCGATTCCGCGCCAATGCGGCAATTGTGGT
TCTACCCAATTAATCACCACCGGCTTAGGCACGGAGCAGTTAGAAGACACCTTAAAAGCGGTTTTCCCAAATTACGGCAT
TGCCCGTATTGACCGTGACACAACGGCGCGCAAAGGAAAATTAGAAGGTTATTTAGCGGATATTCAACAGGGCAAAAGCC
AAATTCTTATCGGGACGCAAATGTTGGCGAAAGGACACCATTTCCCAAATGTCACCTTAGTCGCATTGGTAAATGTGGAT
AGTGCTTTGTTTTCATTGGATTTCCGTGCGGAAGAACGGCTAGCTCAACTTTATATGCAGGTGGCAGGCCGTGCGGGGCG
AGGGGCGCAGCAGGGGGAAGTGTTATTGCAAACCCATTATCCCGAGCATCCGCTATTACAAAGTTTATTGAAGCATGGCT
ATGATAATTTTGCCCAACAAGCATTGGCGTTGCGTCATAAAATGGGATTGCCGCCTTTTAGTTCGCAAGCATTATTTAAA
GCCCAAAGTCGCCATAGCGAGGATGCGGAACAGCTATTGGCGCAGTTTGCCGAGTATTTTTATGGCGTTAAGCAACATAC
TGCACCTGAGTTACAAATTCTCGGTCCTATCACTGCGCCGTTCAGCAAAAAAGCGGGGCAATATCGTTGGCAATTATTGT
TGCAACATCCTTCCCGCCCGATTTTGCAGCAAGTGTTAAATGAATTTCAGACAAGCGAGTTAAATAAACCTGCTCAAGTG
CGGTGGATTTTAGACGTGGATCCGCTAGATTTAGGCTAG

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(504-588)

DEAD/DEAH box helicase (DEAD)

(219-365)

Primosomal protein N C-terminal domain (PriA_C)

(632-728)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

42.683

100

0.43

Multiple sequence alignment