Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   EL257_RS25465 Genome accession   NZ_LR134318
Coordinates   5560633..5562630 (-) Length   665 a.a.
NCBI ID   WP_126367223.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5555633..5567630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS25440 (NCTC9428_05070) fba 5556280..5557344 (-) 1065 WP_008081027.1 class II fructose-bisphosphate aldolase -
  EL257_RS25445 (NCTC9428_05071) - 5557597..5557929 (-) 333 WP_126367216.1 MliC family protein -
  EL257_RS25450 (NCTC9428_05072) - 5557987..5558208 (-) 222 WP_126367218.1 hypothetical protein -
  EL257_RS25455 (NCTC9428_05073) - 5558243..5559406 (-) 1164 WP_126367219.1 phosphoglycerate kinase -
  EL257_RS25460 (NCTC9428_05074) epd 5559419..5560471 (-) 1053 WP_126367221.1 erythrose-4-phosphate dehydrogenase -
  EL257_RS25465 (NCTC9428_05075) recP/tkt 5560633..5562630 (-) 1998 WP_126367223.1 transketolase Machinery gene
  EL257_RS25470 (NCTC9428_05076) - 5562890..5563885 (+) 996 WP_126367226.1 ArsR/SmtB family transcription factor -
  EL257_RS25475 (NCTC9428_05077) metK 5563904..5565094 (+) 1191 WP_034153622.1 methionine adenosyltransferase -
  EL257_RS25480 (NCTC9428_05078) ligB 5565265..5566929 (+) 1665 WP_126367229.1 NAD-dependent DNA ligase LigB -
  EL257_RS25485 (NCTC9428_05079) - 5567027..5567422 (+) 396 WP_126367231.1 DUF1090 domain-containing protein -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 72336.33 Da        Isoelectric Point: 4.8847

>NTDB_id=1001491 EL257_RS25465 WP_126367223.1 5560633..5562630(-) (recP/tkt) [Pseudomonas fluorescens strain NCTC9428]
MPSRRERANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDYLKHNPSNPSFADRDRFVLSNGHGSMLIYSLLHLTGY
DLSIDDLKQFRQLHSRTPGHPEFGYTPGVETTTGPLGQGLANAVGFALAEKVLGAQFNRPGHDIVDHHTYVFLGDGCMME
GISHEVASLAGTLGLGKLIAFYDDNGISIDGEVEGWFTDDTPKRFEAYNWQVIRNVDGHDPEEIKTAIDTARKSAQPTLI
CCKTTIGFGSPNKQGKEDCHGAPLGDAEIALTRQALNWNYGPFEIPADIYAEWDAKEKGRAAEAEWDQRFAAYSAAFPTE
ANELVRRLSGELPADFSEKASAYIAEVAAKGETIASRKASQNTLNAFGPLLPELLGGSADLAGSNLTLWKGCKGVNAEDA
SGNYMYYGVREFGMTAIMNGVTLHGGLVPYGATFLMFMEYARNAVRMSALMKKQVIHVYTHDSIGLGEDGPTHQPIEQLT
SLRTTPNLDTWRPADAVESAVAWKNALERKDGPSALIFSRQNLQHQERDAGQIADISRGGYVLKDCAGEPELILIATGSE
VGLAVQAYDKLTEQGRKVRVVSMPCTSVFDAQDAGYKQAVLPLQVSARIAIEAAHADFWFKYVGLEGRVIGMTTYGESAP
ASALFEEFGFTLENILGQAEELLED

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=1001491 EL257_RS25465 WP_126367223.1 5560633..5562630(-) (recP/tkt) [Pseudomonas fluorescens strain NCTC9428]
ATGCCTAGCCGTCGTGAGCGTGCCAACGCCATTCGTGCCCTCAGCATGGATGCCGTGCAAAAAGCCAACAGCGGTCATCC
CGGTGCCCCTATGGGTATGGCAGATATCGCCGAAGTGCTGTGGCGCGACTACCTCAAGCACAACCCGAGCAATCCTTCGT
TCGCCGACCGTGACCGCTTTGTGCTGTCCAACGGCCACGGCTCGATGTTGATCTATTCGCTGCTGCACCTGACCGGTTAC
GACCTGTCGATCGACGACCTCAAGCAGTTCCGTCAGCTGCACAGCCGCACGCCCGGCCACCCGGAATTCGGTTACACCCC
GGGCGTTGAAACCACCACCGGCCCGCTCGGGCAGGGCCTGGCCAACGCTGTGGGCTTCGCCCTGGCGGAAAAAGTGCTGG
GCGCGCAGTTCAACCGTCCTGGCCACGACATTGTCGATCACCACACCTATGTGTTCCTGGGTGATGGCTGCATGATGGAA
GGCATTTCCCACGAAGTCGCGTCGCTGGCCGGCACCTTGGGTCTGGGCAAGCTGATTGCCTTCTACGATGACAACGGTAT
CTCCATCGACGGCGAAGTCGAAGGCTGGTTCACCGATGACACCCCGAAGCGTTTCGAAGCCTACAACTGGCAGGTCATCC
GCAACGTTGATGGTCACGACCCGGAAGAAATCAAGACTGCCATCGACACCGCGCGCAAAAGCGCCCAGCCAACCCTGATC
TGCTGCAAGACCACCATCGGTTTCGGCTCGCCGAACAAGCAGGGCAAGGAAGACTGCCACGGCGCCCCGCTGGGTGACGC
GGAAATCGCCCTGACCCGTCAGGCACTGAACTGGAATTATGGCCCGTTTGAAATCCCGGCCGACATCTACGCCGAGTGGG
ATGCCAAGGAAAAAGGTCGCGCTGCCGAAGCCGAGTGGGATCAGCGTTTTGCTGCTTACTCCGCCGCGTTCCCGACCGAA
GCCAACGAGCTGGTCCGTCGTCTGAGCGGCGAATTGCCGGCCGACTTCTCCGAAAAAGCCTCGGCTTACATCGCCGAAGT
CGCGGCCAAAGGCGAAACCATCGCCAGCCGCAAAGCCAGCCAGAACACCCTCAACGCATTTGGCCCGCTGCTGCCGGAGC
TGCTCGGCGGTTCCGCTGACCTGGCCGGTTCCAACCTGACCCTGTGGAAAGGTTGCAAAGGCGTCAACGCTGAAGACGCC
AGCGGCAACTACATGTATTACGGCGTGCGCGAATTCGGCATGACCGCGATCATGAACGGCGTGACCCTGCACGGCGGTCT
GGTGCCGTACGGCGCGACCTTCCTGATGTTCATGGAATACGCGCGCAACGCTGTACGCATGTCGGCGCTGATGAAAAAGC
AGGTCATCCACGTTTACACCCACGACTCCATCGGTCTGGGCGAAGACGGCCCGACGCACCAGCCGATCGAGCAACTGACC
AGCCTGCGCACTACACCGAACCTCGACACCTGGCGTCCAGCCGATGCCGTTGAATCGGCGGTGGCCTGGAAAAACGCTCT
GGAGCGCAAGGACGGGCCATCGGCGCTGATTTTCTCGCGTCAGAACCTGCAGCACCAGGAACGCGATGCCGGGCAGATCG
CCGACATCAGCCGCGGCGGTTATGTCCTGAAGGACTGCGCGGGCGAGCCTGAGCTGATCCTGATCGCGACCGGTTCGGAA
GTCGGTCTGGCCGTTCAGGCCTATGACAAACTGACCGAGCAGGGCCGCAAGGTCCGCGTGGTTTCGATGCCATGCACCAG
CGTGTTCGACGCTCAGGATGCCGGCTACAAGCAAGCGGTGCTGCCGTTGCAAGTCAGCGCACGTATCGCGATCGAAGCGG
CCCACGCCGATTTCTGGTTCAAGTACGTGGGTCTGGAAGGTCGCGTGATCGGCATGACTACCTATGGCGAATCGGCCCCG
GCTTCGGCACTGTTCGAAGAGTTCGGCTTCACCCTGGAGAACATTCTGGGTCAGGCTGAAGAGTTGCTGGAAGACTAA

Domains

Predicted by InterProScan.

Transketolase, pyrimidine binding domain (Transket_pyr)

(353-525)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(5-333)

Transketolase, C-terminal domain (Transketolase_C)

(540-655)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.655

99.398

0.474

Multiple sequence alignment