Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EL096_RS00470 Genome accession   NZ_LR134294
Coordinates   89783..92005 (+) Length   740 a.a.
NCBI ID   WP_050971873.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NCTC12977     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 84783..97005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL096_RS00445 (NCTC12977_00088) - 85146..86336 (-) 1191 WP_001865164.1 ferric reductase-like transmembrane domain-containing protein -
  EL096_RS00450 (NCTC12977_00089) - 86585..87244 (-) 660 WP_001864784.1 amino acid ABC transporter permease -
  EL096_RS00455 (NCTC12977_00090) - 87254..87931 (-) 678 WP_001864783.1 amino acid ABC transporter permease -
  EL096_RS00460 (NCTC12977_00091) - 87944..88708 (-) 765 WP_001864782.1 transporter substrate-binding domain-containing protein -
  EL096_RS00465 (NCTC12977_00092) - 88748..89506 (-) 759 WP_001864781.1 amino acid ABC transporter ATP-binding protein -
  EL096_RS00470 (NCTC12977_00093) recJ 89783..92005 (+) 2223 WP_050971873.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EL096_RS00475 (NCTC12977_00094) - 92180..93841 (+) 1662 WP_050221267.1 ribonuclease J -
  EL096_RS00480 (NCTC12977_00095) - 93910..94689 (+) 780 WP_000290025.1 alpha/beta hydrolase -
  EL096_RS00485 (NCTC12977_00096) murM 94801..96021 (+) 1221 WP_000286557.1 peptidoglycan bridge formation alanyltransferase MurM -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 82524.03 Da        Isoelectric Point: 4.3606

>NTDB_id=1000486 EL096_RS00470 WP_050971873.1 89783..92005(+) (recJ) [Streptococcus pneumoniae strain NCTC12977]
MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEE
GENILIYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQVELLDLVAIGTIADMVSLTDENR
ILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKN
EERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDP
HRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKP
IFYIKNFQVESARTMGAGNAHLKLKISKGEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVE
GVQLFNIRGKNTVLPEGVPVLDFPGELPNLAASEAVVVKNIPENITQLKTIFQEQHFSAVYFKNDIDKAYYLTGYGTRDQ
FAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKD
QEMMALGTVQEIYDFLMEKE

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=1000486 EL096_RS00470 WP_050971873.1 89783..92005(+) (recJ) [Streptococcus pneumoniae strain NCTC12977]
TTGATAACACCTACTTATGAATGGCAGTTTGCCCTGCAGGTAGAAGATGCGGATTTTACAAAGATAGCCAAGAAGGCTGG
ACTGGGTCCTGAGGTGGCTCGGTTATTGTTTGAGAGAGGGATTCAGGACCAAGAAAGTCTGAAGAAGTTTTTAGAACCTT
CCTTGGAGGACTTACATGATGCTTATCTGCTCCATGATATGGACAAGGCAGTGGAGCGGATTCGTCAGGCTATTGAAGAA
GGGGAAAATATTCTCATTTATGGAGACTATGATGCGGATGGCATGACTTCGGCTTCTATTGTGAAGGAAAGTTTGGAACA
ACTTGGTGCTGAGTGCCGAGTTTACCTGCCAAATCGTTTTACCGATGGCTATGGCCCTAATGCTAGTGTTTATAAATACT
TTATCGAGCAAGAAGGAATTTCCTTGATTGTGACGGTGGACAATGGGGTTGCTGGTCATGAGGCTATTGCATTGGCTCAG
TCTATGGGAGTAGATGTCATTGTGACAGACCATCATTCCATGCCTGAAACCCTGCCAGATGCTTATGCTATTGTCCATCC
TGAACATCCAGATGCGGATTATCCTTTTAAATATTTGGCTGGTTGTGGAGTTGCTTTCAAGTTGGCTTGTGCCCTGTTAG
AAGAAGTGCAAGTGGAATTGCTTGATTTGGTCGCTATTGGAACTATTGCAGATATGGTGAGTCTGACGGATGAAAATCGT
ATCTTAGTTCAATATGGTCTGGAAATGTTGGGTCATACCCAGCGCATTGGTCTGCAAGAAATGCTGGACATGGCTGGGAT
TGCTGCTAACGAAGTAACAGAAGAAACGGTTGGTTTCCAGATTGCTCCTCGTTTGAATGCCTTGGGCCGCTTGGATGATC
CCAATCCTGCCATTGATTTGTTGACTGGATTTGATGATGAGGAAGCGCATGAGATTGCCCTTATGATTCACCAGAAAAAC
GAAGAGCGTAAGGAAATCGTTCAGTCTATCTATGAAGAAGCCAAGACCATGGTGGATCCTGAGAAGAAGGTTCAGGTCTT
GGCCAAGGAAGGCTGGAATCCTGGGGTTCTAGGAATCGTGGCTGGTCGTTTATTGGAAGAATTGGGACAGACAGTCATTG
TTCTTAATATAGAAGACGGTCGTGCCAAGGGCAGTGCTCGTAGTGTGGAAGCGGTCGATATTTTTGAAGCTCTGGATCCC
CATCGAGACCTCTTCATCGCCTTTGGAGGTCATGCAGGTGCAGCGGGTATGACGCTGGAAGTTGAGCAACTCTCAGATTT
ATCTCAGGTTTTGGAAGATTATGTTCGTGAAAAAGGTGCAGATGCTGGTGGAAAGAATAAGTTAAACCTAGATGAAGAGT
TGGATTTGGAGGCACTTAGCTTGGAAACGGTCAAAAGTTTTGAACGTTTAGCTCCTTTTGGAATGGATAATCAGAAACCT
ATTTTTTATATCAAGAATTTTCAGGTCGAAAGTGCTCGTACTATGGGGGCAGGTAATGCCCATCTAAAGCTGAAAATTTC
CAAGGGTGAGGCGAGTTTTGAAGTGGTAGCCTTTGGTCAAGGCAGATGGGCGACAGAGTTTTCTCAAACCAAGAATCTAG
AGTTAGCGGTTAAATTGTCTGTCAACCAATGGAATGGCCAAACTGCCCTCCAGTTGATGATGGTGGATGCGCGAGTGGAA
GGTGTTCAACTTTTTAACATTCGTGGAAAAAATACAGTCTTGCCAGAAGGTGTTCCAGTCTTGGATTTTCCTGGAGAACT
GCCAAATCTTGCGGCTAGTGAAGCTGTTGTCGTAAAAAACATTCCAGAGAATATTACTCAGCTGAAGACCATTTTTCAGG
AACAGCATTTCTCTGCTGTCTATTTCAAAAATGATATTGACAAGGCCTATTATCTGACAGGTTATGGGACTAGAGATCAG
TTTGCCAAATTGTACAAGACTATTTACCAGTTCCCAGAGTTTGATATTCGCTACAAGCTGAAAGATTTGGCTGCATATCT
TAATATTCAACAAATCTTGCTGGTCAAGATGATTCAAGTATTTGAAGAACTAGGCTTTGTGACGATAAAAGATGGTGTGA
TGACAGTCAATAAAGAGGCGCCAAAGCGGGAGATAGGAGAAAGTCAAATTTACCAAAATCTCAAACAAACCGTTAAAGAC
CAAGAAATGATGGCGCTGGGTACGGTGCAAGAAATTTATGATTTTTTGATGGAAAAAGAGTAG

Domains

Predicted by InterProScan.

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-434)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(593-735)

RecJ OB domain (RecJ_OB)

(451-556)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

99.595

100

0.996
  recJ Bacillus subtilis subsp. subtilis str. 168

37.176

100

0.388