Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QMN06_RS06470 Genome accession   NZ_AP027204
Coordinates   1287398..1288759 (-) Length   453 a.a.
NCBI ID   WP_281969319.1    Uniprot ID   -
Organism   Polynucleobacter sp. SHI8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1282398..1293759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMN06_RS06440 (PSHI8_12590) dapD 1282606..1283445 (+) 840 WP_281969314.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  QMN06_RS06445 (PSHI8_12600) - 1283445..1283810 (+) 366 WP_348649134.1 arsenate reductase -
  QMN06_RS06450 (PSHI8_12610) dapE 1283807..1284976 (+) 1170 WP_281969315.1 succinyl-diaminopimelate desuccinylase -
  QMN06_RS06455 (PSHI8_12620) prmB 1284976..1285863 (+) 888 WP_281969316.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  QMN06_RS06460 (PSHI8_12630) - 1285926..1286915 (+) 990 WP_281969317.1 tripartite tricarboxylate transporter substrate-binding protein -
  QMN06_RS06465 (PSHI8_12640) - 1286959..1287396 (-) 438 WP_281969318.1 thioesterase family protein -
  QMN06_RS06470 (PSHI8_12650) radA 1287398..1288759 (-) 1362 WP_281969319.1 DNA repair protein RadA Machinery gene
  QMN06_RS06475 (PSHI8_12660) - 1288762..1289256 (-) 495 WP_281969320.1 hypothetical protein -
  QMN06_RS06480 (PSHI8_12670) - 1289256..1289600 (-) 345 WP_281969321.1 TIGR01244 family sulfur transferase -
  QMN06_RS06485 (PSHI8_12680) - 1289604..1290809 (-) 1206 WP_281969322.1 O-succinylhomoserine sulfhydrylase -
  QMN06_RS06490 (PSHI8_12690) purF 1290822..1292363 (-) 1542 WP_281969323.1 amidophosphoribosyltransferase -
  QMN06_RS06495 (PSHI8_12700) - 1292381..1292914 (-) 534 WP_281969324.1 CvpA family protein -
  QMN06_RS06500 (PSHI8_12710) - 1292911..1293612 (-) 702 WP_281969325.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48736.33 Da        Isoelectric Point: 7.2442

>NTDB_id=100001 QMN06_RS06470 WP_281969319.1 1287398..1288759(-) (radA) [Polynucleobacter sp. SHI8]
MSKVKSQYVCQSCGASFPKWSGQCAACQEWNTLEEFLEKSSNQRFIGLTKTAPSQKLSQITLEDYGRISTLIPEFDRVLG
GGLVDAGVVLIGGDPGIGKSTLLLQTLASLSQAGESVMYISGEESSAQIALRAQRIGSFAPDLDIYPEILLEKIIPTLEA
VKPRVAVIDSIQTIYSEALSSAPGSVAQVRECASQLTRLAKSSGISILLVGHVTKEGNIAGPRVLEHIVDTVLYFEGDPQ
SSFRLVRAIKNRFGAVNELGVFAMTDKGLKGVSNPSALFLSQSNASVPGTCVLVTQEGTRPLLVEIQALVDQSHVPNPRR
LAVGLEQNRLAMLLAVMHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIQSSLKNKPLPRDLVVFGEVGLAGEIRA
CPRGQERLKEAAKLGFKRAIIPKANLPKQSQFDNMELISVERIETALQIVRDL

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=100001 QMN06_RS06470 WP_281969319.1 1287398..1288759(-) (radA) [Polynucleobacter sp. SHI8]
GTGTCTAAAGTTAAATCTCAGTACGTTTGTCAAAGTTGTGGGGCAAGCTTTCCTAAATGGAGTGGGCAGTGTGCAGCTTG
TCAGGAGTGGAACACACTTGAAGAGTTTTTAGAGAAGTCATCGAATCAGCGCTTTATTGGATTAACTAAAACCGCACCCT
CCCAAAAGTTATCTCAAATCACTCTTGAAGATTACGGTCGAATATCAACCTTAATCCCCGAATTTGATCGCGTATTGGGT
GGCGGACTAGTCGATGCTGGGGTTGTTCTCATTGGTGGCGACCCTGGTATTGGTAAATCAACATTGCTGCTTCAGACACT
GGCCAGTTTAAGCCAAGCTGGCGAGTCAGTGATGTATATCAGTGGCGAGGAATCCTCTGCTCAAATTGCTCTGCGCGCTC
AAAGAATTGGGTCATTTGCCCCAGACTTAGATATTTACCCAGAAATTCTTTTAGAGAAAATCATACCCACATTAGAGGCT
GTAAAGCCTCGAGTTGCAGTCATCGACTCCATCCAAACTATATATTCAGAAGCTTTAAGTTCAGCACCAGGATCAGTCGC
ACAAGTACGAGAGTGCGCATCGCAGTTAACTCGCCTTGCAAAATCTTCTGGTATATCGATACTTCTTGTTGGGCATGTCA
CCAAAGAAGGTAATATTGCAGGCCCTCGTGTTCTTGAGCATATTGTGGATACGGTACTTTATTTTGAAGGTGACCCTCAA
TCTTCATTCCGTTTAGTACGGGCTATAAAAAATCGTTTCGGCGCTGTTAATGAGCTTGGTGTATTTGCGATGACGGATAA
AGGCCTCAAAGGCGTGAGTAATCCATCTGCTTTATTTTTGTCGCAAAGTAATGCTAGTGTTCCTGGAACCTGTGTGTTGG
TAACCCAAGAGGGAACGCGACCTTTATTAGTTGAAATCCAAGCATTAGTAGATCAATCGCATGTACCTAATCCCAGAAGA
TTAGCCGTAGGATTAGAGCAAAATCGATTAGCGATGTTACTTGCAGTCATGCACCGTCATGCAGGAATCGCATGTTTTGA
TCAGGATGTGTTTTTAAATGCGGTAGGTGGGGTAAAAATTACTGAACCTGCAGCCGATCTTGCAGTGCTTCTAGCCATAC
AATCCTCCTTAAAGAATAAACCTTTACCGCGAGATTTAGTGGTATTTGGTGAGGTTGGTTTAGCCGGTGAAATTAGAGCT
TGCCCCCGTGGTCAAGAGCGTTTAAAAGAAGCGGCAAAACTAGGATTTAAGAGAGCAATTATTCCTAAAGCTAACCTTCC
TAAGCAATCTCAATTTGATAATATGGAGTTGATTAGTGTTGAAAGAATTGAGACCGCGCTTCAAATAGTGAGAGATTTGT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(358-425)

AAA domain (AAA_25)

(63-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.78

100

0.499
  radA Streptococcus mitis NCTC 12261

47.137

100

0.472
  radA Streptococcus pneumoniae Rx1

47.137

100

0.472
  radA Streptococcus pneumoniae D39

47.137

100

0.472
  radA Streptococcus pneumoniae R6

47.137

100

0.472
  radA Streptococcus pneumoniae TIGR4

47.137

100

0.472
  radA Streptococcus mitis SK321

47.137

100

0.472

Multiple sequence alignment