Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100179
Name   oriT_pC221 experimental
Organism   Staphylococcus aureus
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_006977 (3084..3160 [+], 77 nt)
oriT length   77 nt
IRs (inverted repeats)    
Location of nic site      67..68
Conserved sequence flanking the
  nic site    		  
Note   _

  oriT sequence  


Download         Length: 77 nt

>oriT_pC221
GGGTATGGGATATAATCCCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACAACGCTTGCCAAACCACA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Varsaki A et al. (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496]
[2] Caryl JA et al. (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]


Relaxosome

This oriT is a component of a relaxosome.

Relaxosome name   RelaxosomepC221 experimental
oriT   oriT_pC221 experimental
Relaxase   MobA_pC221 experimental (_)
Auxiliary protein   mobB_pC221 experimental, mobC_pC221 experimental

  Reference


[1] Caryl JA et al. (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]
[2] Smith MC et al. (2004) An accessory protein is required for relaxosome formation by small Staphylococcal plasmids. J Bacteriol. 186(11):3363-73. [PMID:15150221]
[3] Jamie A Caryl et al. (2004) Reconstitution of a staphylococcal plasmid-protein relaxation complex in vitro. Journal of bacteriology. 186(11):3374-83. [PMID:15150221]


Relaxase

ID   172 GenBank   WP_002493199
Name   MobA_pC221 experimental UniProt ID   P03865
Length   315 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 315 a.a.        Molecular weight: 36445.26 Da        Isoelectric Point: 7.3757

>WP_002493199.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Staphylococcus]
MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKQTRALYGKEDGIQAHTVIQSFKPGEVTPE
QCNQLGLELAEKIAPNHQVAVYTHTDKDHYHNHIVINSVDLETGKKYQSNKKQRDLVKKENDNICREHGL
SVTERGIAKMRYTQAEKGIVFDRDEYSWKDELRDLIENAKTHTSNLETFSEHLEEKGVGVKLRGETISYK
PENENKWVRGRTLGSEYEKGAIDHEHERHQKQQREPEYADEFKINWDAVEQHTEQLKQRRVERAQETKQA
HSKISSRDTRESENQRERAKGNNIRIERGDEGLSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(8-238)


  Protein structure


Source ID Structure
AlphaFold DB P03865

  Reference


[1] Caryl JA et al. (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]


Auxiliary protein

ID   396 GenBank   P03866
Name   mobB_pC221 experimental UniProt ID   P03866
Length   230 a.a. PDB ID   _
Note   

  Auxiliary protein sequence


Download         Length: 230 a.a.        Molecular weight: Da        Isoelectric Point:

>sp|P03866.1|YP2A_STAAU RecName: Full=Uncharacterized protein C
MRKGQLIMSMKDIKNNKENPNTQMNSKSTGTPSSSTQNSLNNEELSELKRQNKLIVKYLAEIQENQKIRE
KEQKEITSELKEATKDFRDKSLKIRNDFVDVLQEKLKHVDTEELKDILGRGIYKVREENDRMLQEVKRSH
EHYQTRQKYLFTGIGAMLLVFMLFALIMTIGSDFMSFLHVDTLQNAIAGKLKASEGFMTFVWYIAYGLPY
VLAIGLFIGLYEWIRAKFHD

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB P03866

ID   397 GenBank   WP_002489523
Name   mobC_pC221 experimental UniProt ID   _
Length   127 a.a. PDB ID   _
Note   

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14626.32 Da        Isoelectric Point: 8.8653

>WP_002489523.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Staphylococcus]
MSEYDNNLASDLSVGENRKPNRKEPKQISFRVSESEYEKLRSSAETLNMSVPNFVKKKAHGSRLVAPKFD
KETRQSIAKDLSKLGANVNQIAKYCNQHQHEAPNYEGLEHNINAVRERLDEIWQQLN

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(80-122)


  Protein structure



No available structure.




Host bacterium

ID   172 GenBank   NC_006977
Plasmid name   pC221 Incompatibility group   -
Plasmid size   4555 bp Coordinate of oriT [Strand]   3084..3160 [+]
Host baterium   Staphylococcus aureus

Cargo genes

Drug resistance gene   cat(pC221)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -