oriTDB
The information of oriT
oriTDB accession number 300232
Name oriT_pKFRT/FLP
oriT length 549 nt
oriT derived from oriT_RP4   [100001]
ATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAG GCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTA CACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCC CTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCAC CGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTC ACCTATCCTGCCCCGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTG GCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGAC CCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTA CCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGAT GCCAAAGAG
D:
Region containing oriT
Reference
[1] Ishikawa M et al (2013) A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination. BMC Microbiol. 0.601388889. [PMID:23594401]
Information of plasmid
ID 445
Plasmid name Suicide vector for unmarked mutagenesis pKFRT/FLP
GenBank accession number AB773262.1
Genome size 6419 bp
Coordinate of oriT  [Strand] 1294..1842
Reference
[1] Ishikawa M et al (2013) A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination. BMC Microbiol. 0.601388889. [PMID:23594401]

Microbial Bioinformatics Group in MML, SJTU