 ID | 7 |
| Name | TrwC_R388  |
| GenBank accession number | FAA00039 |
| Family | MOBF |
| Length | 966 aa |
| UniProt ID | Q2NKF7 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 2.20E-83, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 2.60E-44, Aligned region: 472..659 Viral_helicase1 [PF01443.17], Evalue: 9.50E-07, Aligned region: 492..697 UvrD_C_2 [PF13538.5], Evalue: 1.40E-07, Aligned region: 847..894 |
| Note | relaxase and DNA helicaseactivities |
| Protein sequence [Download] | MLSHMVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLA GNIGEGHRIMRSATRQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQ AEARAQARQKIQGKTRIETTGNLVIGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRAL KNDEIVKRTRYLGAVYNAELAHELQKLGYQLRYGKDGNFDLAHIDRQQIEGFSKRTEQIA EWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQATAKELGIDFSRREWSGR EKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAARLEDIQ KELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWVAELAAKGMKQGAARERVDNAI KTGGLVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREA AELIVSAANRVVGVQGFAGTGKSHMLDTAKQMIEGEGYHVRALAAYGSQVKALRELNVEA NTLASFLRAKDKNIDSRTVLVIDEAGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIE AGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVELAAAGKASSSLERIKDVTEIKNHHER RAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAGKGIEFDTLVRVDTTQA ERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQFSPMTH TKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTD KPLHVDHAYATTVHSSQGLTSDRVLIDAHAESRTTRKDVYYVAISRARFEARVFTNDRGK LPAAIARENIKSAAHDLARDRGGRSAAAERQREQQRERERNRQTQQPAHDRQKAAREAER GMEAGR |
Reference| [1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088] |
| [2] Llosa M et al (1995) Nicking activity of TrwC directed against the origin of transfer of the IncW plasmid R388. J Mol Biol. 246(1):54-62. [PMID:7853404] |
| [3] Llosa M et al (1994) Genetic organization of the conjugal DNA processing region of the IncW plasmid R388. J Mol Biol. 235(2):448-64. [PMID:8289274] |
| [4] de la Cruz F et al (2010) Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiol Rev. 34(1):18-40. [PMID:19919603] |
| [5] Fernández-López R et al (2006) Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution. FEMS Microbiol Rev. 30(6):942-66. [PMID:17026718] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 13 | TrwA_R388 | YP_009182121 | 121 aa | TrwA, together with TrwC and plasmid R388 oriT, forms a stable nucleoprotein structure, the relaxosome |