 oriTDB accession number | 100182 |
| Name | oriT_pTC-F14  |
Sequence Completeness  | core |
| oriT length | 50 nt |
| oriT sequence | GGCTTGGCGGGAACATGCGTAGCATGTGTATGACAAGTCCCATCCTGTTC |
| IR (inverted repeat) | [3-18] [22-37] nt (CTTGGCGGGAACATGC..GCATGTGTATGACAAG) |
| Location of nic site | [47-48] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | _ |
Visualization of oriT structure Reference| [1] Rawlings DE (2005) The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family. Plasmid. 53(2):137-47. [PMID:15737401] |
| [2] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022] |
| ID | 175 |
| Name | MobA_pTC-F14 |
| GenBank accession number | NP_835376 |
| Family | MOBP |
| Length | 889 aa |
| UniProt ID | Q840R3 |
| PDB ID | _ |
| Pfam | RepB_primase [PF16793.4], Evalue: 7.50E-65, Aligned region: 548..809 Relaxase [PF03432.13], Evalue: 4.70E-31, Aligned region: 14..273 |
| Note | relaxase |
| Protein sequence [Download] | MIVKKVKSNRTKGKAASIRDLTNYIREPQNRNPNEKVLYANGRGFISDTHAAQREEMVAL AAEAVRSRNPVNHYILSWREGEQPSPEQVEEAVSIFLDELGLQEHQVIYALHKDTDNLHL HIAVNRVHPETLKCVEINKGFDLESAHRAIARIEHAQGWQREQNGRYEVLENGELGREHL EPNKRRQPEQRKRDKENRTGEKSAERIAIEIGAPIIKQAQSWEQLHRELAAQGMRYEQKG SGALLWVGEVAVKASSADREASLGKLQKRLGAYEPAQAPSPVAQRKPEPLQPDRPEWEDF MAGRKMHYAEKNAAKLSMDQRQEQERKALQARQQEQRKVLLGGRWNGKGEALNALRGVLA AEQAAEKAALKERHQQERQQWRQQYRPYPDFEQWLRREHGAEQATRWRYREAEPQRIEGD STEAPKPRDIRAYRAEIVGQEVRYTPKSGAGGGPGGVSFVDKGRIIEIHDWRNQDTTLAA LQLSAQKWGKFTVTGNDEYKALCVKLAVEHGFQITNPELQEVIRQERQRMRQERAQAMKS EQIKPFERYAEAVGAERYRVTSIKMRPDGSKQTFILDKRDGITRGFTPEEIAQRTPEMQR LQRRGENLYYTPLSEGKHHILIDDMDREKLDRLIRDGYQPAVVLESSPGNYQAVITIPKL GTPFDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHRREDGSYPEVRLLKAE RRECGKTLALSREIDAGYQRQAAEKALKTPVEQFSEPNSTQTTPVSEKTATEAYWRHYRD VRKRQRGMLDLSRVDAMIAVRMRVTGFDQSAIEGAIYQCAPSIREQQESRDWTDYARRTA RYAYSAAGDRQAADLGKYRQQWEKLEGRERQQEQAKAREIERNGPSMSR |
Reference| [1] Rawlings DE (2005) The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family. Plasmid. 53(2):137-47. [PMID:15737401] |
| [2] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 252 | MobB_pTC-F14 | NP_835375 | 103 aa | oriT recognition-like protein |
| 2 | 253 | MobC_pTC-F14 | NP_835374 | 131 aa | histone-like mobilization protein |
| ID | 175 |
| Plasmid name | pTC-F14 |
| GenBank accession number | NC_004734.1 |
| Incompatibility group | IncQ2 |
| Genome size | 14155 bp |
| Coordinate of oriT [Strand] | 2024..2073 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Acidithiobacillus caldus F [33059] |
Reference