oriTDB

The information of the oriT region

oriTDB accession number	100182
Name	oriT_pTC-F14
Sequence Completeness	core
oriT length	50 nt
oriT sequence	GGCTTGGCGGGAACATGCGTAGCATGTGTATGACAAGTCCCATCCTGTTC
IR (inverted repeat)	[3-18] [22-37] nt (CTTGGCGGGAACATGC..GCATGTGTATGACAAG)
Location of nic site	[47-48] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	_
	L:3-18;R:22-37;D:47-48

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCTTGGCGGGAACATGCGTAGCATGTGTATGACAAGTCCCATCCTGTTC

Reference

[1] Rawlings DE (2005) The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family. Plasmid. 53(2):137-47. [PMID:15737401]

[2] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022]

I. Information of Relaxase

ID	175
Name	MobA_pTC-F14
GenBank accession number	NP_835376
Family	MOB_P
Length	889 aa
UniProt ID	Q840R3
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 7.50E-65, Aligned region: 548..809 Relaxase [PF03432.13], Evalue: 4.70E-31, Aligned region: 14..273
Note	relaxase
Protein sequence [Download]	MIVKKVKSNRTKGKAASIRDLTNYIREPQNRNPNEKVLYANGRGFISDTHAAQREEMVAL AAEAVRSRNPVNHYILSWREGEQPSPEQVEEAVSIFLDELGLQEHQVIYALHKDTDNLHL HIAVNRVHPETLKCVEINKGFDLESAHRAIARIEHAQGWQREQNGRYEVLENGELGREHL EPNKRRQPEQRKRDKENRTGEKSAERIAIEIGAPIIKQAQSWEQLHRELAAQGMRYEQKG SGALLWVGEVAVKASSADREASLGKLQKRLGAYEPAQAPSPVAQRKPEPLQPDRPEWEDF MAGRKMHYAEKNAAKLSMDQRQEQERKALQARQQEQRKVLLGGRWNGKGEALNALRGVLA AEQAAEKAALKERHQQERQQWRQQYRPYPDFEQWLRREHGAEQATRWRYREAEPQRIEGD STEAPKPRDIRAYRAEIVGQEVRYTPKSGAGGGPGGVSFVDKGRIIEIHDWRNQDTTLAA LQLSAQKWGKFTVTGNDEYKALCVKLAVEHGFQITNPELQEVIRQERQRMRQERAQAMKS EQIKPFERYAEAVGAERYRVTSIKMRPDGSKQTFILDKRDGITRGFTPEEIAQRTPEMQR LQRRGENLYYTPLSEGKHHILIDDMDREKLDRLIRDGYQPAVVLESSPGNYQAVITIPKL GTPFDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHRREDGSYPEVRLLKAE RRECGKTLALSREIDAGYQRQAAEKALKTPVEQFSEPNSTQTTPVSEKTATEAYWRHYRD VRKRQRGMLDLSRVDAMIAVRMRVTGFDQSAIEGAIYQCAPSIREQQESRDWTDYARRTA RYAYSAAGDRQAADLGKYRQQWEKLEGRERQQEQAKAREIERNGPSMSR

Reference

[1] Rawlings DE (2005) The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family. Plasmid. 53(2):137-47. [PMID:15737401]

[2] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	252	MobB_pTC-F14	NP_835375	103 aa	oriT recognition-like protein
2	253	MobC_pTC-F14	NP_835374	131 aa	histone-like mobilization protein

N/A

Information of plasmid

ID	175
Plasmid name	pTC-F14
GenBank accession number	NC_004734.1
Incompatibility group	IncQ2
Genome size	14155 bp
Coordinate of oriT [Strand]	2024..2073 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acidithiobacillus caldus F [33059]

Reference
N/A