Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4314932..4315563 Replicon chromosome
Accession NZ_CP026803
Organism Shigella flexneri strain 89-141

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C1P88_RS22240 Protein ID WP_001259385.1
Coordinates 4314932..4315207 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C1P88_RS22245 Protein ID WP_000593555.1
Coordinates 4315204..4315563 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P88_RS22225 (4309950) 4309950..4311017 + 1068 WP_000361463.1 HlyD family secretion protein -
C1P88_RS22230 (4311014) 4311014..4313749 + 2736 WP_000149092.1 ribosome-associated ATPase/putative transporter RbbA -
C1P88_RS22235 (4313749) 4313749..4314873 + 1125 WP_001216257.1 ABC transporter permease -
C1P88_RS22240 (4314932) 4314932..4315207 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C1P88_RS22245 (4315204) 4315204..4315563 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1P88_RS22250 (4315683) 4315683..4316084 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C1P88_RS22255 (4316090) 4316090..4316896 - 807 WP_000173669.1 nickel import ATP-binding protein NikE -
C1P88_RS22260 (4316893) 4316893..4317657 - 765 WP_001136237.1 nickel import ATP-binding protein NikD -
C1P88_RS22265 (4317657) 4317657..4318490 - 834 WP_001008975.1 nickel ABC transporter permease subunit NikC -
C1P88_RS22270 (4318487) 4318487..4319431 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4294225..4315563 21338


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T96071 WP_001259385.1 NZ_CP026803:4314932-4315207 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T96071 NZ_CP026803:4314932-4315207 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT96071 WP_000593555.1 NZ_CP026803:4315204-4315563 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT96071 NZ_CP026803:4315204-4315563 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCTTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References