Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 140815..141446 Replicon chromosome
Accession NZ_CP026799
Organism Shigella flexneri strain NCTC 9728

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C1P82_RS00705 Protein ID WP_001259385.1
Coordinates 140815..141090 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C1P82_RS00710 Protein ID WP_000593555.1
Coordinates 141087..141446 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P82_RS00690 135833..136900 + 1068 WP_000361470.1 HlyD family secretion protein -
C1P82_RS00695 136897..139632 + 2736 WP_000149171.1 ribosome-associated ATPase/putative transporter RbbA -
C1P82_RS00700 139632..140756 + 1125 WP_001216257.1 ABC transporter permease -
C1P82_RS00705 140815..141090 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C1P82_RS00710 141087..141446 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1P82_RS00715 141566..141967 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C1P82_RS00720 141973..142779 - 807 WP_000173670.1 nickel import ATP-binding protein NikE -
C1P82_RS00725 142776..143540 - 765 WP_001136248.1 nickel import ATP-binding protein NikD -
C1P82_RS00730 143540..144373 - 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
C1P82_RS00735 144370..145314 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 120411..141446 21035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T96013 WP_001259385.1 NZ_CP026799:140815-141090 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T96013 NZ_CP026799:140815-141090 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT96013 WP_000593555.1 NZ_CP026799:141087-141446 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT96013 NZ_CP026799:141087-141446 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References