Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1493390..1494021 Replicon chromosome
Accession NZ_CP026793
Organism Shigella flexneri strain 74-1170

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag C1P81_RS07465 Protein ID WP_001259384.1
Coordinates 1493746..1494021 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C1P81_RS07460 Protein ID WP_000593555.1
Coordinates 1493390..1493749 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P81_RS07435 (1489522) 1489522..1490466 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
C1P81_RS07440 (1490463) 1490463..1491296 + 834 WP_134799818.1 nickel ABC transporter permease subunit NikC -
C1P81_RS07445 (1491296) 1491296..1492060 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
C1P81_RS07450 (1492057) 1492057..1492863 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
C1P81_RS07455 (1492869) 1492869..1493270 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C1P81_RS07460 (1493390) 1493390..1493749 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1P81_RS07465 (1493746) 1493746..1494021 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
C1P81_RS07470 (1494080) 1494080..1495204 - 1125 WP_001216257.1 ABC transporter permease -
C1P81_RS07475 (1495204) 1495204..1497939 - 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
C1P81_RS07480 (1497936) 1497936..1499003 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1493390..1513152 19762


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T95972 WP_001259384.1 NZ_CP026793:c1494021-1493746 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T95972 NZ_CP026793:c1494021-1493746 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT95972 WP_000593555.1 NZ_CP026793:c1493749-1493390 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT95972 NZ_CP026793:c1493749-1493390 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References