Detailed information of TA system TA095824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3294972..3295603
• Replicon: chromosome
• Accession: NZ_CP026776
• Organism: Shigella flexneri strain 98-3193

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C1P84_RS16990
• Protein ID: WP_025760846.1
• Coordinates: 3294972..3295247 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: C1P84_RS16995
• Protein ID: WP_000593555.1
• Coordinates: 3295244..3295603 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P84_RS16975 (3289990)	3289990..3291057	+	1068	WP_000361470.1	HlyD family secretion protein	-
C1P84_RS16980 (3291054)	3291054..3293789	+	2736	WP_000149172.1	ribosome-associated ATPase/putative transporter RbbA	-
C1P84_RS16985 (3293789)	3293789..3294913	+	1125	WP_001216257.1	ABC transporter permease	-
C1P84_RS16990 (3294972)	3294972..3295247	+	276	WP_025760846.1	type II toxin-antitoxin system HicA family toxin	Toxin
C1P84_RS16995 (3295244)	3295244..3295603	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C1P84_RS17000 (3295723)	3295723..3296124	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
C1P84_RS17005 (3296130)	3296130..3296936	-	807	WP_000173660.1	nickel import ATP-binding protein NikE	-
C1P84_RS17010 (3296933)	3296933..3297697	-	765	WP_001136248.1	nickel import ATP-binding protein NikD	-
C1P84_RS17015 (3297697)	3297697..3298530	-	834	WP_001008974.1	nickel ABC transporter permease subunit NikC	-
C1P84_RS17020 (3298527)	3298527..3299471	-	945	WP_000947076.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T Genomic island TA120378 4222298..4243335 0.002	inside	Genomic island	-	-	3273790..3295603	21813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-81)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10145.95 Da
• Isoelectric Point: 10.6469

>T95824 WP_025760846.1 NZ_CP026776:3294972-3295247 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T95824 NZ_CP026776:3294972-3295247 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGAAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT95824 WP_000593555.1 NZ_CP026776:3295244-3295603 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT95824 NZ_CP026776:3295244-3295603 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6