Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3172039..3172565 Replicon chromosome
Accession NZ_CP026612
Organism Escherichia coli strain DTU-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C4J63_RS16280 Protein ID WP_000323025.1
Coordinates 3172039..3172326 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C4J63_RS16285 Protein ID WP_000534858.1
Coordinates 3172326..3172565 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J63_RS16240 3167063..3167278 - 216 WP_000839590.1 phage lysis protein EssD -
C4J63_RS23315 3167498..3167668 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C4J63_RS16245 3168032..3168247 - 216 WP_000066484.1 cold shock-like protein CspB -
C4J63_RS16250 3168548..3168760 + 213 WP_000087756.1 cold shock-like protein CspF -
C4J63_RS23050 3168815..3168904 + 90 WP_120795389.1 hypothetical protein -
C4J63_RS16255 3169182..3169934 - 753 WP_001047135.1 antitermination protein -
C4J63_RS16260 3169948..3170997 - 1050 WP_001265198.1 DUF968 domain-containing protein -
C4J63_RS16265 3170999..3171277 - 279 WP_012304870.1 hypothetical protein -
C4J63_RS16270 3171344..3171595 - 252 WP_000980994.1 hypothetical protein -
C4J63_RS16275 3171812..3171967 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C4J63_RS16280 3172039..3172326 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C4J63_RS16285 3172326..3172565 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C4J63_RS16290 3172590..3172895 + 306 WP_001326990.1 hypothetical protein -
C4J63_RS16295 3173098..3173430 + 333 WP_001301033.1 protein FlxA -
C4J63_RS23055 3173867..3174016 - 150 WP_011443592.1 hypothetical protein -
C4J63_RS16305 3174051..3174329 - 279 Protein_2960 hypothetical protein -
C4J63_RS16310 3174313..3174543 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C4J63_RS16315 3174627..3175034 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C4J63_RS16320 3175201..3175356 + 156 WP_000379575.1 DUF1391 family protein -
C4J63_RS16330 3175516..3175734 + 219 WP_001171942.1 hypothetical protein -
C4J63_RS16340 3176302..3176490 + 189 WP_000854559.1 cell division inhibition protein DicB -
C4J63_RS16345 3176487..3176678 + 192 WP_001083297.1 lysis protein YdfD -
C4J63_RS16350 3176771..3177538 + 768 Protein_2967 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3159040..3179401 20361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T95275 WP_000323025.1 NZ_CP026612:c3172326-3172039 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T95275 NZ_CP026612:c3172326-3172039 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT95275 WP_000534858.1 NZ_CP026612:c3172565-3172326 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT95275 NZ_CP026612:c3172565-3172326 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References