95275

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3172039..3172565 Replicon chromosome
Accession NZ_CP026612
Organism Escherichia coli strain DTU-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C4J63_RS16280 Protein ID WP_000323025.1
Coordinates 3172039..3172326 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C4J63_RS16285 Protein ID WP_000534858.1
Coordinates 3172326..3172565 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J63_RS16240 3167063..3167278 - 216 WP_000839590.1 phage lysis protein EssD -
C4J63_RS23315 3167498..3167668 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C4J63_RS16245 3168032..3168247 - 216 WP_000066484.1 cold shock-like protein CspB -
C4J63_RS16250 3168548..3168760 + 213 WP_000087756.1 cold shock-like protein CspF -
C4J63_RS23050 3168815..3168904 + 90 WP_120795389.1 hypothetical protein -
C4J63_RS16255 3169182..3169934 - 753 WP_001047135.1 antitermination protein -
C4J63_RS16260 3169948..3170997 - 1050 WP_001265198.1 DUF968 domain-containing protein -
C4J63_RS16265 3170999..3171277 - 279 WP_012304870.1 hypothetical protein -
C4J63_RS16270 3171344..3171595 - 252 WP_000980994.1 hypothetical protein -
C4J63_RS16275 3171812..3171967 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C4J63_RS16280 3172039..3172326 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C4J63_RS16285 3172326..3172565 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C4J63_RS16290 3172590..3172895 + 306 WP_001326990.1 hypothetical protein -
C4J63_RS16295 3173098..3173430 + 333 WP_001301033.1 protein FlxA -
C4J63_RS23055 3173867..3174016 - 150 WP_011443592.1 hypothetical protein -
C4J63_RS16305 3174051..3174329 - 279 Protein_2960 hypothetical protein -
C4J63_RS16310 3174313..3174543 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C4J63_RS16315 3174627..3175034 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C4J63_RS16320 3175201..3175356 + 156 WP_000379575.1 DUF1391 family protein -
C4J63_RS16330 3175516..3175734 + 219 WP_001171942.1 hypothetical protein -
C4J63_RS16340 3176302..3176490 + 189 WP_000854559.1 cell division inhibition protein DicB -
C4J63_RS16345 3176487..3176678 + 192 WP_001083297.1 lysis protein YdfD -
C4J63_RS16350 3176771..3177538 + 768 Protein_2967 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3159040..3179401 20361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T95275 WP_000323025.1 NZ_CP026612:c3172326-3172039 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T95275 NZ_CP026612:c3172326-3172039 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT95275 WP_000534858.1 NZ_CP026612:c3172565-3172326 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT95275 NZ_CP026612:c3172565-3172326 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References