Detailed information of TA system TA095235

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1718618..1718838
• Replicon: chromosome
• Accession: NZ_CP026598
• Organism: Clostridioides difficile strain 10-00253

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: C3352_RS08345
• Protein ID: WP_003429855.1
• Coordinates: 1718618..1718779 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1718699..1718838 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3352_RS08310	1714475..1714912	+	438	WP_009888839.1	hypothetical protein	-
C3352_RS08315	1714905..1715081	+	177	WP_009888840.1	hypothetical protein	-
C3352_RS08320	1715082..1716392	+	1311	WP_009893136.1	phage tail sheath family protein	-
C3352_RS08325	1716409..1716879	+	471	WP_009888842.1	phage tail tube protein	-
C3352_RS08330	1716938..1717765	+	828	WP_009888843.1	hypothetical protein	-
C3352_RS08335	1717837..1718277	+	441	WP_003429853.1	phage portal protein	-
C3352_RS08345	1718618..1718779	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1718699..1718838	-	140	NuclAT_1	-	Antitoxin
-	1718699..1718838	-	140	NuclAT_2	-	Antitoxin
-	1719183..1719231	-	49	NuclAT_4	-	-
-	1719183..1719231	-	49	NuclAT_5	-	-
C3352_RS08350	1720116..1720304	+	189	WP_003429858.1	hypothetical protein	-
C3352_RS08355	1720423..1721289	+	867	WP_009888845.1	hypothetical protein	-
C3352_RS08365	1722154..1722681	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
C3352_RS08370	1722819..1723586	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T95235 WP_003429855.1 NZ_CP026598:1718618-1718779 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T95235 NZ_CP026598:1718618-1718779 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCACAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT95235 NZ_CP026598:c1718838-1718699 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin