Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1645346..1645872 Replicon chromosome
Accession NZ_CP026353
Organism Escherichia coli strain 4A

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C2R10_RS08635 Protein ID WP_000323025.1
Coordinates 1645346..1645633 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C2R10_RS08640 Protein ID WP_000534858.1
Coordinates 1645633..1645872 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R10_RS08595 1640370..1640585 - 216 WP_000839590.1 phage lysis protein EssD -
C2R10_RS24440 1640805..1640975 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C2R10_RS08600 1641339..1641554 - 216 WP_000066484.1 cold shock-like protein CspB -
C2R10_RS08605 1641855..1642067 + 213 WP_000087756.1 cold shock-like protein CspF -
C2R10_RS24165 1642122..1642211 + 90 WP_120795389.1 hypothetical protein -
C2R10_RS08610 1642489..1643241 - 753 WP_001047135.1 antitermination protein -
C2R10_RS08615 1643255..1644304 - 1050 WP_001393597.1 DUF968 domain-containing protein -
C2R10_RS08620 1644306..1644584 - 279 WP_012304870.1 hypothetical protein -
C2R10_RS08625 1644651..1644902 - 252 WP_000980994.1 hypothetical protein -
C2R10_RS08630 1645119..1645274 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C2R10_RS08635 1645346..1645633 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C2R10_RS08640 1645633..1645872 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C2R10_RS08645 1645897..1646202 + 306 WP_001326990.1 hypothetical protein -
C2R10_RS08650 1646405..1646737 + 333 WP_001301033.1 protein FlxA -
C2R10_RS24170 1647174..1647323 - 150 WP_011443592.1 hypothetical protein -
C2R10_RS08660 1647358..1647636 - 279 Protein_1583 hypothetical protein -
C2R10_RS08665 1647620..1647850 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C2R10_RS08670 1647934..1648341 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C2R10_RS08675 1648508..1648663 + 156 WP_000379575.1 DUF1391 family protein -
C2R10_RS08685 1648823..1649041 + 219 WP_001171942.1 hypothetical protein -
C2R10_RS08695 1649609..1649797 + 189 WP_000854559.1 cell division inhibition protein DicB -
C2R10_RS08700 1649794..1649985 + 192 WP_001083297.1 lysis protein YdfD -
C2R10_RS08705 1650078..1650845 + 768 Protein_1590 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1614804..1669592 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T94334 WP_000323025.1 NZ_CP026353:c1645633-1645346 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T94334 NZ_CP026353:c1645633-1645346 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT94334 WP_000534858.1 NZ_CP026353:c1645872-1645633 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT94334 NZ_CP026353:c1645872-1645633 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References