Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3932930..3933645 Replicon chromosome
Accession NZ_CP026098
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name yafO Uniprot ID A0A658YZ27
Locus tag MC63_RS20360 Protein ID WP_001263492.1
Coordinates 3932930..3933349 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag MC63_RS20365 Protein ID WP_000554759.1
Coordinates 3933352..3933645 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS20335 (3928233) 3928233..3929690 + 1458 WP_025759252.1 cytosol nonspecific dipeptidase -
MC63_RS20340 (3929747) 3929747..3930307 - 561 Protein_3885 peptide chain release factor H -
MC63_RS20345 (3930423) 3930423..3931467 - 1045 Protein_3886 RNA ligase RtcB family protein -
MC63_RS20350 (3931713) 3931713..3932165 - 453 WP_001059862.1 GNAT family N-acetyltransferase -
MC63_RS20360 (3932930) 3932930..3933349 - 420 WP_001263492.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MC63_RS20365 (3933352) 3933352..3933645 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MC63_RS20370 (3933697) 3933697..3934752 - 1056 WP_001226172.1 DNA polymerase IV -
MC63_RS20375 (3934823) 3934823..3935608 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
MC63_RS20380 (3935580) 3935580..3937292 + 1713 Protein_3893 flagellar biosynthesis protein FlhA -
MC63_RS20385 (3937509) 3937509..3938006 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3909016..3938736 29720
flank IS/Tn - - 3932199..3932702 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16348.95 Da        Isoelectric Point: 8.0871

>T93308 WP_001263492.1 NZ_CP026098:c3933349-3932930 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRFWVMLPTY

Download         Length: 420 bp

>T93308 NZ_CP026098:c3933349-3932930 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGGG
TAATGCTGCCAACTTACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT93308 WP_000554759.1 NZ_CP026098:c3933645-3933352 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT93308 NZ_CP026098:c3933645-3933352 [Shigella flexneri]
ATGCATCGGATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A658YZ27

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References