Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1643370..1643896 Replicon chromosome
Accession NZ_CP026026
Organism Escherichia coli strain CAR

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C2H81_RS08625 Protein ID WP_000323025.1
Coordinates 1643370..1643657 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C2H81_RS08630 Protein ID WP_000534858.1
Coordinates 1643657..1643896 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2H81_RS08585 1638394..1638609 - 216 WP_000839590.1 phage lysis protein EssD -
C2H81_RS23940 1638829..1638999 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C2H81_RS08590 1639363..1639578 - 216 WP_000066484.1 cold shock-like protein CspB -
C2H81_RS08595 1639879..1640091 + 213 WP_000087756.1 cold shock-like protein CspF -
C2H81_RS23670 1640146..1640235 + 90 WP_120795389.1 hypothetical protein -
C2H81_RS08600 1640513..1641265 - 753 WP_001047135.1 antitermination protein -
C2H81_RS08605 1641279..1642328 - 1050 WP_001393597.1 DUF968 domain-containing protein -
C2H81_RS08610 1642330..1642608 - 279 WP_012304870.1 hypothetical protein -
C2H81_RS08615 1642675..1642926 - 252 WP_000980994.1 hypothetical protein -
C2H81_RS08620 1643143..1643298 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C2H81_RS08625 1643370..1643657 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C2H81_RS08630 1643657..1643896 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C2H81_RS08635 1643921..1644226 + 306 WP_001326990.1 hypothetical protein -
C2H81_RS08640 1644429..1644761 + 333 WP_001301033.1 protein FlxA -
C2H81_RS23675 1645198..1645347 - 150 WP_011443592.1 hypothetical protein -
C2H81_RS08650 1645382..1645660 - 279 Protein_1582 hypothetical protein -
C2H81_RS08655 1645644..1645874 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C2H81_RS08660 1645958..1646365 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C2H81_RS08665 1646532..1646687 + 156 WP_000379575.1 DUF1391 family protein -
C2H81_RS08675 1646847..1647065 + 219 WP_001171942.1 hypothetical protein -
C2H81_RS08685 1647633..1647821 + 189 WP_000854559.1 cell division inhibition protein DicB -
C2H81_RS08690 1647818..1648009 + 192 WP_001083297.1 lysis protein YdfD -
C2H81_RS08695 1648102..1648869 + 768 Protein_1589 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1629026..1650732 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T92762 WP_000323025.1 NZ_CP026026:c1643657-1643370 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T92762 NZ_CP026026:c1643657-1643370 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT92762 WP_000534858.1 NZ_CP026026:c1643896-1643657 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT92762 NZ_CP026026:c1643896-1643657 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References