Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28569..29212 Replicon plasmid pOXA10_020023
Accession NZ_CP025944
Organism Escherichia coli strain SCEC020023

Toxin (Protein)


Gene name vagD Uniprot ID A0A7W3HBH1
Locus tag C1A20_RS00175 Protein ID WP_044864680.1
Coordinates 28796..29212 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag C1A20_RS00170 Protein ID WP_001261276.1
Coordinates 28569..28799 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1A20_RS00140 23723..24499 - 777 WP_000015958.1 site-specific integrase -
C1A20_RS00145 24496..25239 - 744 WP_000129823.1 hypothetical protein -
C1A20_RS00150 25290..25640 - 351 WP_000493378.1 hypothetical protein -
C1A20_RS00160 26034..27107 + 1074 WP_077890912.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
C1A20_RS00165 27400..28314 + 915 WP_052686739.1 Abi family protein -
C1A20_RS00170 28569..28799 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C1A20_RS00175 28796..29212 + 417 WP_044864680.1 type II toxin-antitoxin system VapC family toxin Toxin
C1A20_RS00180 29421..30437 - 1017 WP_001310555.1 IS5-like element IS5 family transposase -
C1A20_RS00185 30602..33667 - 3066 WP_000002334.1 tetrathionate reductase subunit TtrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / tet(A) / floR / sul2 - 1..89313 89313
flank IS/Tn - - 29421..30401 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.64 Da        Isoelectric Point: 8.5403

>T92527 WP_044864680.1 NZ_CP025944:28796-29212 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T92527 NZ_CP025944:28796-29212 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACAAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT92527 WP_001261276.1 NZ_CP025944:28569-28799 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT92527 NZ_CP025944:28569-28799 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCAGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3HBH1

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References