Detailed information of TA system TA089621

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2661615..2661824
• Replicon: chromosome
• Accession: NZ_CP025047
• Organism: Clostridioides difficile strain W0003a

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: CWR54_RS12440
• Protein ID: WP_004454815.1
• Coordinates: 2661666..2661824 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2661615..2661749 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR54_RS12425	2657052..2658680	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
CWR54_RS12430	2658801..2659796	+	996	WP_003431031.1	asparaginase	-
CWR54_RS12435	2659974..2660357	-	384	Protein_2355	BlaI/MecI/CopY family transcriptional regulator	-
-	2661615..2661749	+	135	NuclAT_0	-	Antitoxin
CWR54_RS12440	2661666..2661824	-	159	WP_004454815.1	hypothetical protein	Toxin
CWR54_RS12445	2662118..2663488	-	1371	WP_021373596.1	cell wall-binding protein Cwp29	-
CWR54_RS12450	2663704..2664821	+	1118	Protein_2358	site-specific integrase	-
CWR54_RS12455	2665184..2665264	-	81	Protein_2359	VOC family protein	-
CWR54_RS12460	2665304..2665972	-	669	WP_021361938.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T89621 WP_004454815.1 NZ_CP025047:c2661824-2661666 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T89621 NZ_CP025047:c2661824-2661666 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT89621 NZ_CP025047:2661615-2661749 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin