Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2661615..2661824 Replicon chromosome
Accession NZ_CP025047
Organism Clostridioides difficile strain W0003a

Toxin (Protein)


Gene name CD2517.1 Uniprot ID Q182K5
Locus tag CWR54_RS12440 Protein ID WP_004454815.1
Coordinates 2661666..2661824 (-) Length 53 a.a.

Antitoxin (RNA)


Gene name RCd8
Locus tag -
Coordinates 2661615..2661749 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWR54_RS12425 2657052..2658680 + 1629 WP_004454813.1 aspartate 4-decarboxylase -
CWR54_RS12430 2658801..2659796 + 996 WP_003431031.1 asparaginase -
CWR54_RS12435 2659974..2660357 - 384 Protein_2355 BlaI/MecI/CopY family transcriptional regulator -
- 2661615..2661749 + 135 NuclAT_0 - Antitoxin
CWR54_RS12440 2661666..2661824 - 159 WP_004454815.1 hypothetical protein Toxin
CWR54_RS12445 2662118..2663488 - 1371 WP_021373596.1 cell wall-binding protein Cwp29 -
CWR54_RS12450 2663704..2664821 + 1118 Protein_2358 site-specific integrase -
CWR54_RS12455 2665184..2665264 - 81 Protein_2359 VOC family protein -
CWR54_RS12460 2665304..2665972 - 669 WP_021361938.1 VanZ family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-128)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5996.20 Da        Isoelectric Point: 11.3102

>T89621 WP_004454815.1 NZ_CP025047:c2661824-2661666 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Download         Length: 159 bp

>T89621 NZ_CP025047:c2661824-2661666 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin


Download         Length: 135 bp

>AT89621 NZ_CP025047:2661615-2661749 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V849

Antitoxin

Download structure file

References