Detailed information of TA system TA089612

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3598696..3598911
• Replicon: chromosome
• Accession: NZ_CP025046
• Organism: Clostridioides difficile strain W0022a

Toxin (Protein)

• Gene name: CD0977.1
• Uniprot ID: Q18AH6
• Locus tag: CWR55_RS16585
• Protein ID: WP_011861054.1
• Coordinates: 3598768..3598911 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	3598696..3598842 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR55_RS16560	3594154..3594978	-	825	WP_003432350.1	phosphate ABC transporter permease PstA	-
CWR55_RS16565	3594980..3595897	-	918	WP_021361136.1	phosphate ABC transporter permease subunit PstC	-
CWR55_RS16570	3596278..3596724	-	447	WP_021361137.1	hypothetical protein	-
CWR55_RS16575	3597447..3597833	-	387	WP_021366416.1	BlaI/MecI/CopY family transcriptional regulator	-
CWR55_RS16580	3597873..3598154	-	282	WP_070538156.1	BlaI/MecI/CopY family transcriptional regulator	-
-	3598696..3598842	+	147	NuclAT_3	-	Antitoxin
-	3598696..3598846	+	151	NuclAT_0	-	-
CWR55_RS16585	3598768..3598911	-	144	WP_011861054.1	hypothetical protein	Toxin
CWR55_RS16590	3599172..3599360	+	189	WP_009898401.1	hypothetical protein	-
CWR55_RS16595	3599608..3600312	-	705	WP_011861053.1	hypothetical protein	-
CWR55_RS16600	3600314..3600745	-	432	WP_011861052.1	protein-export chaperone SecB	-
CWR55_RS16605	3600763..3601326	-	564	WP_021371073.1	hypothetical protein	-
CWR55_RS16610	3601872..3602168	-	297	WP_021371072.1	hypothetical protein	-
CWR55_RS16615	3602185..3603000	-	816	WP_021371071.1	N-acetylmuramoyl-L-alanine amidase	-
CWR55_RS16620	3603000..3603257	-	258	WP_021366402.1	phage holin family protein	-
CWR55_RS16625	3603261..3603551	-	291	WP_021363500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3595881..3650048	54167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5337.27 Da
• Isoelectric Point: 10.5719

>T89612 WP_011861054.1 NZ_CP025046:c3598911-3598768 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKLNKKQH

Nucleotide

• Length: 144 bp

>T89612 NZ_CP025046:c3598911-3598768 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTGAAAATCAAGCTTAATAAAAAACAACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT89612 NZ_CP025046:3598696-3598842 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTT
GTTTTTTATTAAGCTTGATTTTCACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: Q18AH6

Antitoxin