Detailed information of TA system TA089609

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2662198..2662407
• Replicon: chromosome
• Accession: NZ_CP025046
• Organism: Clostridioides difficile strain W0022a

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: CWR55_RS12655
• Protein ID: WP_004454815.1
• Coordinates: 2662249..2662407 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2662198..2662332 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR55_RS12640	2657825..2659453	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
CWR55_RS12645	2659574..2660569	+	996	WP_003431031.1	asparaginase	-
CWR55_RS12650	2660758..2661141	-	384	Protein_2400	BlaI/MecI/CopY family transcriptional regulator	-
-	2662198..2662332	+	135	NuclAT_4	-	Antitoxin
CWR55_RS12655	2662249..2662407	-	159	WP_004454815.1	hypothetical protein	Toxin
CWR55_RS12660	2662766..2664136	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
CWR55_RS12665	2664419..2664517	+	99	Protein_2403	Arm DNA-binding domain-containing protein	-
CWR55_RS12670	2664536..2664957	+	422	Protein_2404	hypothetical protein	-
CWR55_RS12675	2665188..2665460	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
CWR55_RS12680	2665778..2666446	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T89609 WP_004454815.1 NZ_CP025046:c2662407-2662249 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T89609 NZ_CP025046:c2662407-2662249 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT89609 NZ_CP025046:2662198-2662332 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin